不同浓度的银杏内酯B对培养的神经干细胞分化的影响

THE EFFECTS OF GINKGOLID B OF VARIOUS CONSISTENCY ON THEDIFFERENTIATION OF NEURAL STEM CELLS

目的 探讨不同浓度的银杏内酯B对神经干细胞分化的影响。 方法 从新生大鼠海马中分离和培养神经干细胞。将其接种在 2 4孔培养板中 ,并分成 4组 :2 0mg L内酯B组、4 0mg/L内酯B组、6 0mg/L内酯B组和对照组。 4组神经干细胞被分别加入含不同浓度银杏内酯B的DMEM/F12培养液 ,在培养 7d和 14d后 ,用免疫细胞化学染色方法检测其神经丝蛋白 (NF)、胶质原纤维酸性蛋白 (GFAP)及少突胶质细胞特异性蛋白 (Oligo)的表达 ,并计算阳性染色细胞的百分率。 结果 在同一时间内不同浓度银杏内酯B组分化为NF阳性神经元样细胞的百分率都比对照组高 ,但不同浓度之间没有显著差异。在同一时间内不同浓度银杏内酯B对少突胶质样细胞的百分率影响不大。而不同浓度银杏内酯B组分化为GFAP阳性星形胶质样细胞的百分率都比对照组高 ,并随其浓度的增加而百分率增高。 结论 不同浓度的银杏内酯B可促进神经干细胞向神经元样细胞分化 ,但与其浓度关系不大 ;对少突胶质样细胞的百分率影响不大 ;对星形胶质样细胞的百分率有促进作用 ,并随浓度的增加而增高。

Objective To observe the effects of Ginkgolid B of various consistency on the differentiation of neural stem cells. Methods Neural stem cells(NSCs) were dissociated and cultured from the hippocampus of newborn rat.The NSCs plated into 24 well cultured plates were divided into 20mg/L,40mg/L,60mg/L Ginkgolid B groups and control group.Four groups of NSCs were cultured respectively using DMEM/F12 containing Ginkgolid B of various consistency.At 7 days and 14 days after NSCs cultured, NF, GFAP and Oligodendrocyte special protein(Oligo) expression were detected using immunocytochemical stains respectively.The numbers of NF,GFAP and Oligo positive cells were counted.The percentages of positive cells were obtained respectively. Results The percentage of NF positive neuron-like cells in the various consistency of Ginkgolid B groups was significantly higher than that in the control group,but there were no significantly differents among the various consistency of Ginkgolid B group.The effect of Ginkgolid B of various consistency on NSCs differentiation of Oligo positive cells was insignificant.The percentage of GFAP positive cells in the various consistency of Ginkgolid B groups was significantly higher than that in the control group,and the percentage was increasing with the consistencies of Ginkgolid B.Conclusion Ginkgolid B may enhance NSCs differentiate into NF positive neuron-like cells,but it is no relation to the consistency of Ginkgolid B.Ginkgolid B may not promote NSCs differentiate into oligodendrocyte-like cells.Ginkgolid B may enhance NSCs differentiate into GFAP positive astroglia-like cells and the percentage of GFAP positive cells were increased with the consistencies of Ginkgolid B.