appps1双转基因阿尔茨海默病模型小鼠脑内胶质细胞的激活及iNOS的表达

ACTIVATION OF NEUROGLIA CELLS AND EXPRESSION OF iNOS IN THE BRAINS OF app/ps1 DOUBLE TRANSGENIC MOUSE

目的 研究 10～ 12月龄雌性app ps1双转基因阿尔茨海默病 (AD)模型小鼠 (app ps1,dtg)脑内小胶质细胞和星形胶质细胞的激活 ,以及诱导型一氧化氮合酶 (induciblenitricoxide,iNOS)的表达。 方法 免疫组织化学结合刚果红组织学染色 ,普通光学显微镜观察。 结果 在app ps1dtg小鼠皮质和海马内有广泛的神经炎斑形成 ,围绕在神经炎斑周围的小胶质细胞和星形胶质细胞处于活化状态 ,且活化的星形胶质细胞表达iNOS。 结论 app ps1dtg小鼠能够模拟AD病人脑内的主要病理过程。神经胶质细胞的激活及iNOS的表达在app ps1dtg小鼠和AD脑内病理过程中具有重要的作用。

Objective To examine the expression of inducible nitric oxide synthase(iNOS) and activation of neuroglia cells in the brain of app/ps1 double transgenic mouse(app/ps1 dtg). Methods The brain sections from 6 app/ps1 dtg and 3 wild type female mice aged at 10 to 12 month were subjected to immunohistochemistry and Congo red histological staining,and observed under microscope. Results The widespread neuritic plaques were observed in the hippocampus and cerebral cortex of app/ps1 dtg mice.These plaques were surrounded by activated microglia cells and astrocytes,and the activated astrocytes expressed iNOS.Conclusion The findings in the present study indicate that app/ps1 dtg mice could mimic the main pathological changes similar to those in Alzheimer disease(AD),suggesting neuroglia activation and expression of iNOS play an important role in app/ps1 dtg mice and AD.