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鸡实验性骨折愈合过程TGF-β1免疫组织化学研究 被引量：1

IMMUNOHISTOLOGICAL PATTERN OF TGF-β1 IN CHICKENCALLUS IN EXPERIMENTAL FRACTURE HEALING
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摘要 目的　探索鸡实验性骨折愈合过程中骨折局部TGF β1及其含量变化。 　方法　 16只 6月龄新罗曼母鸡 ,无菌手术条件下制造右肱骨中部一致性骨折模型 ,取骨折局部 3、6、10和 15d的组织材料 ,采用SABC法对TGF β1进行检测。 　结果　SABC染色显示骨折后 3d ,TGF β1阳性物出现于血肿的细胞中 ;6dTGF β1阳性反应出现在结缔组织细胞膜上、胶原纤维和细胞外基质中 ;10d、15dTGF β1阳性物存在于软骨细胞外基质 ,与骨折后 3d相比 ,6d、10d、15dTGF β1的分布明显增加 (P <0 0 1)。 　结论　TGF β1的表达与鸡骨折愈合过程密切相关。 Objective To observe transforming growth factor-β1(TGF-β1) in chicken callus. Methods The right humerus fracture models of 16 New Roman hens, at the age of 6-month old, about 1.75kg in weight, were established under no germ operational condition. TGF-β1 of the tissue samples procured from callus on 3d,6d,10d and 15d of fracture healing were examined by immunohistochemical method. Results Histochemical study shows that the immunostaining of TGF-β1 exists in blood cells on 3d, connective tissue cells and extracellular matrix on 6d, cartilage on 10d and 15d. Compared with that on 3d, the expression of TGF-β1 increased markedly on 6d, 10d and 15d of fracture healing(P<0.01).Conclusion TGF-β1 plays an important role in fracture healing in chicken.
作者 董常生 贺俊平 高贵民
机构地区 山西农业大学动物科技学院 山西省人民医院
出处 《解剖学报》 CAS CSCD 北大核心 2004年第5期534-536,共3页 Acta Anatomica Sinica
关键词 骨折愈合 转化生长因子-Β1 免疫组织化学 鸡 Fracture healing TGF-β1 Immunohistochemistry Chicken
分类号 R683 [医药卫生—骨科学]
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参考文献9

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同被引文献21

  • 1张玮,王少安.转化生长因子-β1与种植体骨整合的研究进展[J].国际口腔医学杂志,2008,35(S1):250-252. 被引量：3
  • 2Bomstein MM, Lussi A, Schmid B, et al. Early load- ing of nonsubmerged titanium implants with a sand- blasted and acid-etched (SLA) surface:3-year re- sults of a prospective study in partially edentulous patients [J] .Int J Oral Maxillofac Implants, 2003, 18 (5) :659-666.
  • 3O' Driscoll SW, Fitzsimmons JS. Clin Orthop Relat Res, 2001, (391 Suppl) : S190- 207.
  • 4Lamberg A, Schmidmaler G, Soballe K. Locally de- livered TGFbetal and IGF-1 enhance the fixation of titanium implants: A study in dogs [J] .Acta Or- thop, 2006, 77 (5): 799-805.
  • 5Tielinen L, Puolakkainen P, Pohjonen T.The effect of transforruing growth factorβ1, released from a bioabsorbable self-reinforced polylactide pin, on a bone defect [J] .Biomaterials, 2002, 23 (18) : 3817-3823.
  • 6Vehof JW, Hans MT, de Ruijter AE, et al.Bone formation in Transform ing Growth Factor betal loaded titanium fiber mesh implants [J] .Clin Oral Impl Res, 2002, 13 (1): 94-102.
  • 7Nikolidakis, D, et al. The effect of a low dose of transforming growth factor betal (TGF-beta1) on the early bone-healing around oral implants inserted in trabecular bone. Biomatefials, 2009, 30(1): 94-9.
  • 8Tang Y, et al. TGF-beta1-induced migration of bone mesenchymal stem ceils couples bone resorp- tion with formation. Nat Med, 2009, 15 (7) : 757- 65.
  • 9Pietruski JK, Pietmska MD, Stokowska W, et al. Evaluation ofpolypeptide growth factors in the pro- cess of dental implant osseointegration [J].Rocz Akad Med Bialymst, 2001, 46 (1): 19.27.
  • 10Akinci B, et al. Low transforming growth factor-be- tal serum levels in idiopathic male osteoporosis. J Endocrinol Invest, 2007, 30 (5) : 350-5.

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解剖学报
2004年 第5期

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