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人可溶性B淋巴细胞刺激因子结合小肽的筛选及其免疫抑制功能分析

Screening of small peptides binding to human soluble B-lymphocyte stimulator and the analysis of their immunosuppressive effects
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摘要 目的筛选和鉴定B淋巴细胞刺激因子(BLyS)的抑制性小肽。方法用重组人BLyS筛选噬菌体构象型7肽库,人工合成小肽鉴定其免疫抑制功能。结果经过筛选,得到两个阳性噬菌体克隆,人工合成的两个小肽在体外能特异性地抑制BLyS刺激细胞增生的活性。结论获得了两个能够抑制BlyS功能的小肽。 Objective To screen and characterize an antagonistic peptide of B-lymphocyte stimulator(BLyS). Methods C7C phage display peptide library was screened with BLyS. The peptides were synthesized by FMOC method.The suppressive effects of the synthetic peptides to the avtivation of lymphocytes by BlyS were assayed. Results After screening, two phage clones were obtained. The peptides synthesized could inhibit the BLyS activity in vitro. Conclusion Two antagonistic peptides of BLyS are obtained.
作者 吉清 郑英如 李蓉芬 钟小林 高会广 何凤田
机构地区 第三军医大学生物化学与分子生物学教研室 第三军医大学大坪医院妇产科
出处 《中华风湿病学杂志》 CAS CSCD 2004年第11期641-643,共3页 Chinese Journal of Rheumatology
基金 基金项目:国家自然科学基金资助项目(30271228 30370319 30400187)
关键词 B淋巴细胞刺激因子 免疫抑制 筛选 噬菌体 刺激细胞 体克 体外 结论 目的 方法 Peptide library B-lymphocyte stimulator Peptide Immunosuppression
分类号 R392 [医药卫生—免疫学] R593 [医药卫生—内科学]
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参考文献10

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二级参考文献23

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共引文献11

中华风湿病学杂志
2004年 第11期

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