阿西美辛在高锰酸钾亚硫酸钠体系中的流动注射化学发光分析

Flow injection chemiluminescent detection of acemetacin in KMnO_4-Na_2SO_3 system

目的 研究阿西美辛对高锰酸钾 亚硫酸钠化学发光体系的增敏作用 ,建立阿西美辛的简便、快速测定新方法。方法 利用阿西美辛对高锰酸钾 亚硫酸钠化学发光体系的增敏作用 ,结合流动注射技术对其含量进行定量测定。结果 在 1 0× 10 - 2 mol·L- 1 H3PO4 - 5 0× 10 - 5mol·L- 1 KMnO4 - 4 0× 10 - 4mol·L- 1 Na2 SO3体系中 ,化学发光强度与阿西美辛浓度在 1 0× 10 - 7- 1 0× 10 - 5mol·L- 1 呈线性关系 ,在 3倍信噪比 ,阿西美辛的检出限为 6 9× 10 - 8mol·L- 1 ,对 2 5× 10 - 6 mol·L- 1 阿西美辛测定获得满意结果。结论 该方法快速、简便、准确度好、灵敏度高、选择性好 ,对临床医学研究具有实际意义。

Aim To study the sensitizing effect of acemetacin (ACE) on the weak chemiluminescent (CL) reaction of KMnO 4 with sulfite and establish a fast and convenient method for CL detection of ACE. Methods Using the sensitizing effect of ACE on KMnO 4-Na 2SO 3 system and flow injection technique to determine the concentration of ACE. Results Under optimal conditions, the CL intensity of 1.0×10 -2 mol·L -1 H 3PO 4 - 5.0×10 -5 mol·L -1 KMnO 4 - 4.0×10 -4 mol·L -1 Na 2SO 3 was proportional to the concentration of ACE ranging from 1.0×10 -7 to 1.0×10 -5 mol·L -1. The detection limit of ACE was 6.9×10 -8 mol·L -1 at 3σ. Satisfactory results were obtained for determination of ACE at 2.5×10 -6 mol·L -1. Conclusion The present method showed good precision,high sensitivity and selectivity and could be used for fast and convenient detection of ACE. It would be of significance to the clinical and pharmacological study of acemetacin.