上皮性卵巢癌中PTEN和Ki-67的表达及意义

Expression and significance of pten and KI-67 in ovarian epithelial carcinoma

目的 探讨上皮性卵巢癌中PTEN和Ki- 6 7的蛋白表达及意义。方法 采用免疫组织化学S -P法检测了 6 4例上皮性卵巢癌 ,30例卵巢良性肿瘤和 2 0例正常卵巢组织中PTEN和Ki- 6 7的蛋白表达。分析PTEN和Ki- 6 7的蛋白表达及其与各种临床病理参数、预后的关系及二者的相关性。结果 在上皮性卵巢癌组织中PTEN蛋白的阳性率明显低于正常卵巢组织和卵巢良性肿瘤组织。PTEN蛋白在病理分化差的G3组阳性率明显低于分化较好的G1-G2组 ;在临床Ⅲ～Ⅳ期组PTEN蛋白阳性率明显低于临床Ⅰ～Ⅱ期组。而Ki- 6 7在以上各组的阳性率与PTEN蛋白阳性率相比恰好相反。PTEN和Ki- 6 7蛋白的阳性率与年龄、组织类型均无关。在上皮性卵巢癌中 ,PTEN与Ki- 6 7的蛋白表达呈负相关 (r=- 0 5 94 )。患者的生存时间与PTEN蛋白阳性率呈正相关 ,与Ki- 6 7阳性率呈负相关。结论 PTEN蛋白在上皮性卵巢癌中表达明显低于正常及良性肿瘤组织 ,提示PTEN的缺失与卵巢癌的发生、发展有关 ;PTEN、Ki- 6 7阳性率与上皮性卵巢癌的病理分化、临床分期和生存时间有关 ,提示PTEN蛋白表达的缺失和减少导致细胞增殖的增加 ,预示着卵巢癌的不良预后。

Objective To investigate the expression of PTEN and Ki-67 in ovarian epithelial carcinoma and their relationship with clinicopathologic factors and prognosis.Methods The expression of PTEN and Ki-67 in 64 cases of ovarian epithelial cancer, 30 benign tumors and 20 normal ovarian tissue were determined by S-P immunohistochemical method.Results The positive rate of PTEN in the ovarian cancer tissue was lower than in the normal and benign ovarian tissues. The expression level of PTEN in stage Ⅲ～Ⅳ group was lower than that of stage Ⅰ～Ⅱ group (P<0.05); in poorly differentiated cancer (G3) was lower than well differentiated (G1-G2). Ki-67 expression trend was opposite to that of PTEN. No different expression of PTEN and Ki-67 among histological types or ages was found. In the ovarian cancer, negative correlation between expression of PTEN and Ki-67 was observed (r=-0.594). In univariate analysis, the expression of PTEN and Ki-67 were associated with survival rate. Conclusions PTEN expression in ovarian cancer was down-regulated. The expression level of PTEN and Ki-67 were associated with differentiation, clinic stage and survival. The relationship between PTEN and Ki-67 suggests that PTEN may suppress the tumor through down-regulate cell proliferation.