周围型肺腺癌增强CT与血管生成的相关研究

The Correlation Study between Enhanced CT and Angiogenesis in Peripheral Lung Adenocarcinoma

目的 探讨周围型肺腺癌CT增强表现与血管生成的关系。材料与方法 对 2 9例周围型肺腺癌标本进行S P法免疫组织化学染色 ,检测VEGF表达 ,并且用FVⅢ RA标记微血管内皮细胞来检测微血管密度 (MVD)。全部病例CT增强的同时有 17例进行了 3min内的动态CT增强扫描。结果 2 9例周围型肺腺癌组织中的MVD与血管内皮生长因子 (VEGF)呈正相关 (r=0 .76 ) ,有淋巴结转移组的MVD、VEGF强表达率明显高于无淋巴结转移组(P <0 .0 5 ) ,Ⅱ期组的MVD、VEGF强表达率明显高于Ⅰ期组 (P <0 .0 1) ;其中 17例 3min内的CT增强值与MVD呈正相关 (r=0 .73～ 0 .84 ) ;VEGF强表达组的CT增强值显著高于弱表达组 (P <0 .0 5 )。结论 周围型肺腺癌在3min内的CT增强值与血管生成呈正相关 。

Objective To study the relationship between enhanced CT findings and angiogenesis in peripheral adenocarcinoma.Materials and Methods VEGF expression level of tumor specimens were estimated with immunohistochemical SP method in 29 cases with peripheral lung adenocarcinoma. The microvessel density of lung cancer estimated by counting epithelial cells marked by FVⅢ-RA. Dynamic CT in 3 minutes was performed in 17 cases among all CT enhanced cases. Results The MVD was correlated positively (r=0.76) with VEGF in 29 cases of peripheral adenocarcinoma. The group with nodule metastases had higher MVD and percentage of VEGF++/+++ than that of without metastases (P<0.05), and group of stage Ⅱ had higher MVD and percentage of VEGF++/+++ than that of stage Ⅰ (P<0.01). Among those, the CT enhanced value within 3 minutes was correlated with MVD positively in 17 cases (r=0.73～0.84), the CT enhanced value of VEGF++/+++ group was higher than that of VEGF-/+ group significantly (P<0.05).Conclusion The enhanced value of peripheral lung adenocarcinoma in 3 minutes was correlated positively with angiogenesis, and may indicate the degree of malignant of lung cancer.