脑胶质瘤的致性与侵袭性的相关研究

Relationship between invasibility and epileptogenesis of glioma in human

目的 探讨胶质瘤所致癫与不同级别胶质瘤侵袭性的相关作用及其临床意义。方法 根据临床资料 ,将胶质瘤所致癫组 5 5例病人定为实验组 ,其他非胶质瘤所致癫组 38例病人定为对照组。实验组又根据病理分型分为良性组 37例和恶性组 18例。放免技术测定血清LN及IV型胶原含量 ,流式细胞仪技术测定 93例患者肿瘤与正常脑组织交界处 1cm× 2cm× 2cm区域的CD4 4含量及DNA倍体含量分析 ,高效液相色谱仪测定交界处脑组织的GABA浓度。结果 恶性胶质瘤组 (18/ 5 5 )交界处标本中CD4 4含量为 5 0 3%± 1 2 3% ,与良性胶质瘤组(37/ 5 5 )CD4 4含量 3 4 7%± 0 80 %及对照组CD4 4含量 1 93%± 0 90 %相比有显著差异 (q1=3 95 ,q2 =4 5 2 ,P <0 0 5 ) ,恶性肿瘤组GABA浓度明显高于对照组 (P <0 0 5 ) ,而良性组则低于对照组 (P <0 0 5 )。恶性胶质瘤组患者血清中LN、IV型胶原含量与对照组相比有显著差异 (P <0 0 5 ) ,但与良性胶质瘤组患者无明显差异 (P >0 0 5 )。 5 5例胶质瘤患者术后LN和Ⅳ型胶原含量全部下降 ,手术效果有显著差异 (P <0 0 5 )。恶性胶质瘤所致癫组 18例患者中有 15例DNA倍体呈异倍体 ,细胞增殖活跃。良性胶质瘤组 37例中仅 3例出现异倍体 ,大多呈二倍体。

Objective To investigate the relationship between the invasibility and epileptogenesis of glioma and its clinic significance. Methods 93 patients with epilepsy caused by brain tumors of glioma were classified as the study group(55 patients with glioma) and the nonglioma group(38 patients). The levels of LN and Collagen Ⅳ in serum were measured by radioimmunoassay in all patients. Meanwhile, CD44 and GABA in 2 cm 2 peripheral areas around the tumors and were measured by FCM and HPLC. The analysis of DNA was performed to decide the degree of invasibility. Results CD44 and GABA in samples with malignant glioma were much higher than those with benign glioma or with non glioma tumors( P <0 05). The levels of LN and Collagen Ⅳ in serum were much higher in patients with malignant glioma than those in the control group( P <0 05), whereas not significantly different from those in patients with benign glioma, and diploid chromasome in the majority of patients with benign glioma. Conclusions The lower the invasibility of glioma was, the higher the epilep togenesis would be. The change of GABA concentration played an important role in the invasion of glioma. The results also illustrated that abnormality of DNA in glioma was closely relate with its epileptogenesis.