肿瘤坏死因子的基因型与扩张型心肌病的相关性研究

Influence of TNF-α gene polymorphism on dilated cardiomyopathy

目的探讨中国北方地区汉族人肿瘤坏死因子鄄α(TNF鄄α)基因型别的多态性与扩张型心肌病(DCM)的相关性。方法对26例DCM患者采用放射免疫分析法(RIA)测定TNF鄄α,应用顺序特异性引物聚合酶链反应(PCR鄄SSP)方法检测TNF鄄α基因型别的多态性变化,并与31名健康献血者对比。结果DCM组TNF鄄α(1.986±0.101)显著高于对照组(1.06±0.061),P<0.01;DCM组TNF鄄α(308A)等位基因频率(39.26%)显著高于对照组(11.76%),P<0.01。提示该等位基因频率增高与DCM相关。结论北方地区汉族人TNF鄄α基因308位点的多态性与DCM的易感性相关联。

Objective To study the relationships between TNF-α genotype in Northern Chinese and dilated cardio myopathy(DCM). Methods TNF-α genotypes polymorphism were analyzed by Polymerase Chain Reaction-Special Sequence Primer (PCR-SSP) in twenty-six DCM patients, and thirty-one controls. Serum TNF-α levels were determined by RIA. Results The TNF-α levels were higher in DCM patients than controls (P < 0.01); The frequency of TNF-α (308A) genotype was significantly higher in DCM patients than that of controls(P < 0.01). The TNF-α(308A) genotype had significant influence on DCM. Conclusions TNF-α(308A) genotype in Northern Chinese positively relates to DCM and most likely contributes to high risk for DCM.