水溶性金属卟啉的合成、表征及其清除有毒活性氧的研究

Synthesis and anti-active oxygen properties of water-soluble metal porphyrins

目的 合成并结构表征了4种水溶性金属卟啉配合物[5,10,15,20-四[4-(4’-吡啶-1)丁氧基苯基]金属卟啉溴化盐[锌卟啉(Ⅰ)、铜卟啉(Ⅱ)、锰卟啉(Ⅲ)和钴卟啉(Ⅳ)],作为抗活性氧(O2-,H2O2,HO-)模拟酶。方法核黄素-蛋氨酸光照测其清除O2-作用,H2O2氧化Vit C法测其催化H2O2的分解,Fenton-苯甲酸钠荧光法测其对HO-清除作用,小鼠肝匀浆法测其抗脂质过氧化作用。结果 1.0×10-5-1.0×10-6mol·L-1均具有良好的清除O2-作用;1.5×10-6-1.0×10-6mol·L-1均具有分解H2O2作用;2.0×10-8-1.0×10-8mol·L-1均具有清除HO-的功能;1.0×10-7mol·L-1均可使脂质过氧化产物明显减少。结论 合成4种水溶性金属卟啉配合物可作为抗多种活性氧(O2-,H2O2,HO-)模拟酶。

Aim To synthesize four water-soluble metal porphyrins [5, 10, 15 , 20-tetra [ 4-( 4'-pyridine-1) butyloxy phenyl] metalloporphyrins bromide, metal = Zn (Ⅰ), Cu (Ⅱ), Mn (Ⅲ) and Co (Ⅳ)] as analogous enzyme having two anti-active oxygen functions. Methods The first function, scavenging O2 , has been proved by using riboflavine-methionine photoreduction methods. The second function, scavenging H2O2, has been demonstrated by using the oxidating Vit C. The third function, scavenging HO , has been demonstrated by using Fenton reaction. The complexes were measured by the mice liver homogenate technique of mice. Results Four model compounds could scavenge O2-in the concentration range of 1.0×10-5-1.0×10-6mol · L-1, decompose H2O2 in the concentration of 1. 5 x 10-6 -1.0×10-6Vol· L-1, scavenge HO' in the concentration of 2. 0×10-8 -1.0×10-8mol·L-1. All showed that they had obvious action of decreasing the lipid peroxidation in the concentration of 1.0×10-7 mol·L-1. Conclusion All above-mentioned complexes were considered to be qualified analogous enzymes of anti-active oxygen.