摘要
The structural and functional study of protein is a major topic of current functional genomics. Fluorescence resonance energy transfer (FRET) is one of few tools available for measuring nanometer scale distances and changes in distances in vivo . FRET is an ideal technology for detection of protein conformation and protein-protein interaction by using fluorescence protein, traditional organic dyes and other dyes as probes. It uses fluorescence protein, traditional organic dyes and other dyes as its probes. The application of FRET in the determination of intracellular events would be helpful for us to understand the structure and function of biology molecules. [
The structural and functional study of protein is a major topic of current functional genomics. Fluorescence resonance energy transfer (FRET) is one of few tools available for measuring nanometer scale distances and changes in distances in vivo . FRET is an ideal technology for detection of protein conformation and protein-protein interaction by using fluorescence protein, traditional organic dyes and other dyes as probes. It uses fluorescence protein, traditional organic dyes and other dyes as its probes. The application of FRET in the determination of intracellular events would be helpful for us to understand the structure and function of biology molecules. [
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2004年第9期1721-1724,1738,共5页
Chinese Journal of Pathophysiology
基金
国家杰出青年科学基金资助项目(No.39925014)
国家自然科学基金资助项目(No.30030060
No.39800074
No.39870332)
关键词
荧光共振能量转移
蛋白质相互作用
Fluorescence resonance energy transfer
Protein interaction
