大鼠缺血脑中差异表达基因的研究

Differential expression genes in the rat ischemic brain

目的鉴定大鼠脑缺血差异表达基因。方法复制大鼠大脑中动脉栓塞急性脑缺血模型,采用荧光差异显示RT-PCR法结合反Northern杂交快速鉴定差异表达基因。结果发现差异表达的基因9个,其中6个已知的序列和3个未知序列;已知基因中上调的是musmusculusab1-interactor1,homosapiensCGI-99protein,tissueinhibitorofmetalloproteinase3,homosapiensnuclearreceptorco-repressor,下调的是homo-sapiensnuclearmatrixproteinp84和coatomerproteincomplex,subunitgamma2。结论荧光差异显示法结合反Northern杂交是一种有效的快速鉴定差异表达基因的方法;脑缺血时,缺血侧和非缺血侧存在基因的差异表达。

AIM: To confirm the differential expression genes in the rat ischemic brain. METHODS: The middle cerebral artery occlusion ischemic model was set up in rats. Fluorescence differential display reverse transcriptase-polymerase chain reaction (FDD RT-PCR) and reverse Northern blotting were used to fast confirm the differential expression genes. RESULTS: Nine differential expression sequence tags, including 6 known sequences and 3 unknown sequences, were confirmed. Among the known sequences, mus musculus ab1-interactor1,homo sapiens CGI-99 protein, tissue inhibitor of metalloproteinase 3 and homosapiens nuclear receptor co-repressor were up-regulated while homo-sapiens nuclear matrix protein p84 and coatomer protein complex, subunit gamma 2 were down-regulated. CONCLUSIONS: ① Combination of fluorescence differential display reverse transcriptase-polymerase chain reaction (FDD RT-PCR) and reverse Northern blotting is a method to fast-confirm the differential expression genes; ② There are differential expression genes in ischemic brain regions compared to non-ischemic parts. [