参麦注射液对Ang Ⅱ诱导培养心肌细胞凋亡的影响

Shenmai injection protects rat cardiomyocytes from angiotensin Ⅱ-induced apoptosis in vitro

目的观察参麦注射液对血管紧张素-Ⅱ(AngⅡ)诱导培养心肌细胞凋亡的影响。方法乳鼠心肌细胞原代培养,MTT法观察参麦注射液对培养心肌细胞活力的影响;荧光染色、流式细胞仪检测细胞凋亡,Fluo-3荧光探针标记测定钙离子荧光强度。结果AngⅡ(10-7mol/L)孵育48h后心肌细胞凋亡明显多于对照组(P<005);参麦注射液(05g/L、10g/L)组心肌细胞活力显著高于AngⅡ组(P<005);参麦注射液(10g/L)组AngⅡ诱导的心肌细胞凋亡率明显低于AngⅡ组(P<005),AngⅡ所导致的心肌细胞内钙超载明显轻于AngⅡ组(P<001)。结论参麦注射液对AngⅡ诱导的细胞凋亡具有明显的抑制作用,其机制可能与其抑制心肌细胞内钙超载有关。

AIM: To study the protective effects of Shenmai injection, a Chinese medicine, on angiotensin Ⅱ (AngⅡ)-induced rat cardiomyocyte apoptosis in vitro and the probable mechanism. METHODS: Cultured cardiomyocytes from neonatal rats were stimulated with AngⅡ. Cell viability were measured by MTT, and apoptosis was evaluated suing Hoethst33258 fluorescent dye staining and flow cytometry. Fluo-3/AM was used to test the change in intracellular free calcium. RESULTS: It was found that incubation with AngⅡ (10 -7 mol/L) for 48 h increased cardiomyocyte apoptosis, Shenmai injection (0 5 g/L, 1 0 g/L) inceased myocyte viability ( P<0 05 ). Shenmai injection (1 0 g/L) significantly decreased the AngⅡ-induced rat cardiomyocyte apoptosis ( P<0 05 ) and decreased fulorescent intensity of intracellular calcium. CONCLUSION: Shenmai injection has a significant inhibitory effect on AngⅡ-induced rat cardiomycoyte apoptosis in vitro by alleviating intracellular calcium overload. [