养血清脑血清拮抗溶血磷脂酸诱导大鼠血管平滑肌细胞增殖

Yangxue qingnao-containing serum inhibits rat vascular smooth muscle cell proliferation induced by lysophosphatidic acid

目的观察养血清脑药物血清对溶血磷脂酸(LPA)诱导的大鼠血管平滑细胞(VSMC)增殖作用的影响。方法在培养的大鼠平滑肌细胞上,测定[3H]-胸腺嘧啶核苷([3H]-thymidine,[3H]-TdR)掺入、丝裂素活化蛋白激酶(mitogen-activatedproteinkinase,MAPK)活性及脂质过氧化终产物丙二醛(malonyldialdehyde,MDA)含量。结果1×10-9、1×10-8和1×10-7mol·L-1LPA呈浓度依赖性引起VSMC[3H]-TdR掺入量、MAPK活性及MDA含量增加。5%、10%和15%养血清脑血清预孵育使1×10-7mol·L-1LPA诱导VSMC[3H]-TdR掺入量分别下降230%、420%和520%(P<001);使VSMCMAPK的活性分别下降139%(P<005)、296%(P<001)和489%(P<001);使细胞MDA含量分别下降194%、247%和432%(P<001)。结论LPA呈浓度依赖性引起VSMC增殖及脂质过氧化,VSMC增殖与其细胞内信号转导MAPK途径有关。养血清脑血清可有效地拮抗LPA诱导的VSMC增殖、MAPK激活和脂质过氧化损伤。

AIM: To observe the effects of Yangxue qingnao-containing serum on rat vascular smooth muscle cell (VSMC) proliferation induced by lysophosphatidic acid (LPA). METHODS: The [ 3H]-TdR incorporation and mitogen-activated protein kinasc (MAPK) activity were measured in cultured VSMC. End product of lipid peroxidation-MDA levels were also detected. RESULTS: 1×10 -9 ,1×10 -8 and 1×10 -7 mol/L LPA enhanced the cultured VSMC [ 3H]-TdR incorporation, increased MAPK activity and MDA content in a concentration-dependent manner. 5%, 10% and 15% Yangxue qingnao-containing serum concentration-dependently inhibited the increase in VSMC [ 3H]-TdR incorporation, MAPK activity and MDA content induced by LPA. CONCLUSIONS: LPA has a stimulating effect on VSMC proliferation. The LPA-induced intracellular signal transduction may be related to MAPK activity. Yangxue qingnao can efficiently inhibit LPA -induced VSMC proliferation,MAPK activity and lipid peroxidation. [