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雌激素对大鼠血管平滑肌细胞囊泡素-1基因表达的影响 被引量：1

Effect of estrogen on the gene expression of caveolin-1 in rat vascular smooth muscle cells
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摘要 目的探讨雌激素对血管平滑肌细胞囊泡素-1基因表达的影响。方法取Wistar雌性大鼠,分为3组假手术组,卵巢切除后皮下埋植雌激素组(OVX+E组)及卵巢切除后皮下埋植安慰剂组(OVX+V组)。用药2周后处死大鼠,剥离主动脉平滑肌组织,提取总RNA进行半定量RT-PCR分析,检测雌激素对囊泡素-1(caveolin-1)基因表达的影响。为进一步明确雌激素是否直接调节血管平滑肌细胞caveolin-1基因表达,又采用100nmol/L17β-雌二醇(17β-E2)处理培养的大鼠血管平滑肌细胞24h,通过Northernblot分析检测雌激素对细胞caveolin-1mRNA表达的影响。结果OVX+E组大鼠主动脉平滑肌组织caveolin-1基因表达量明显高于OVX+V组,17β-E2处理的培养细胞中caveolin-1基因mRNA表达量高于未用药的培养细胞。结论雌激素可促进血管平滑肌细胞caveolin-1基因表达,反映了雌激素心血管作用机理的一个方面。 AIM: To assess the effect of estrogen on the gene expression of caveolin-1 in rat vascular smooth muscle cells (VSMCs). METHODS: Wistar rats were ovariectomized and subjected to subcutaneous implantation of placebo pellets (OVX+V group) or estradiol pellets (OVX+E group). 2 weeks after implantation, the expression of caveolin-1 gene in endothelium-denuded aortic tissue was examined by RT-PCR. Furthermore, Northern blotting was used to analyze the mRNA expression of caveolin-1 in cultured rat VSMCs. RESULTS: RT-PCR showed that expression of caveolin-1 gene was significantly higher in OVX+E group than that in OVX+V group. Northern blot analysis showed that the mRNA expression of caveolin-1 was higher in VSMCs pretreated with 17β-estradiol (17β-E 2) than that in VSMCs without 17β-E 2 pretreatment. CONCLUSION: Estrogen up-regulates the gene expression of caveolin-1 in the vascular wall, partially indicating the cardiovascular effect of estrogen. [
作者 杨发林 刘贤锡 贺红 张建业 许伟华 张岩
机构地区 山东大学医学院生物化学和分子生物学研究所 齐鲁医院心内科 齐鲁医院第二医院内科
出处 《中国病理生理杂志》 CAS CSCD 北大核心 2004年第9期1638-1641,共4页 Chinese Journal of Pathophysiology
关键词 雌激素 细胞质膜微囊蛋白 血管平滑肌细胞 Estrogen Caveolins Vascular smooth muscle cells
分类号 R363 [医药卫生—病理学]
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参考文献10

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同被引文献17

  • 1Lisabeth LD, Beiser AS, Brown DL, et al. Age at natural menopause and risk of ischemic stroke the Framingham heart study[J]. Stroke,2009 ;40 (4) :1044-6.
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  • 9马晓华,毕宏生,李镜海,解孝锋,吴建峰,季鹏.雌二醇对牛视网膜毛细血管内皮细胞VEGF、HIF-1α的调控[J].山东大学耳鼻喉眼学报,2008,22(1):6-9. 被引量：2
  • 10刘轲,李建生,周友龙,高剑峰,杨歆科,赵跃武,刘政国,刘敬霞.老龄大鼠脑缺血/再灌注微血管生成及VEGF/VEGFR系统表达变化[J].中国老年学杂志,2010,30(20):2931-2935. 被引量：4

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中国病理生理杂志
2004年 第9期

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