EB病毒潜伏膜蛋白1对鼻咽癌细胞P53蛋白表达的影响

Effect of Epstein-Barr virus latent membrane protein 1 on expression of P53 protein in nasopharyngeal carcinoma cells

目的研究EB病毒潜伏膜蛋白1(LMP1)对鼻咽癌细胞P53蛋白表达的影响。方法将LMP1基因真核表达质粒转染至鼻咽癌CNE1细胞,脂质体介导端粒酶反义核酸处理转染细胞,MTT法检测细胞增殖能力,免疫组化法检测LMP1和P53蛋白表达,原位杂交技术检测端粒酶逆转录酶(hTERT)mRNA表达。结果对照组,转染并表达LMP1基因的细胞的增殖能力、P53蛋白和hTERTmRNA表达水平均显著高于未转染细胞和转染空载质粒的细胞。端粒酶反义核酸作用组,LMP1基因转染细胞的LMP1蛋白表达水平显著低于对照组(P<001);LMP1基因转染细胞与未转染细胞和转染空载质粒的细胞的增殖能力、P53蛋白和hTERTmRNA表达水平均显著低于对照组(P<001),但LMP1基因转染细胞的增殖能力和P53蛋白表达水平仍显著高于未转染细胞和转染空载质粒的细胞(P<001)。结论EB病毒LMP1可促进鼻咽癌细胞P53蛋白的表达。

AIM: To study the effect of Epstein-Barr virus(EBV) latent membrane protein 1(LMP1) on expression of P53 protein in nasopharyngeal carcinoma(NPC) cells. METHODS: EBV-LMP1 gene expression plasmid was transfected into NPC CNE1 cells. After being treated with telomerase antisense oligodeoxynucleotide (asODN), the proliferation of transfected cells was identified by MTT method, and the expression of LMP1 and P53 protein and human telomerase reverse transcriptase (hTERT) mRNA was detected by immunohistochemistry and in situ hybridization technique, respectively. RESULTS: In control group, the proliferate ability and the expression of P53 protein and hTERT mRNA of LMP1-transfected cells were significantly higher than those of untransfected cells and vector-transfected cells ( P< 0 01). The expression of LMP1 protein in LMP1-transfected cells were significently lower in asODN groups than in control groups ( P< 0 01); The proliferate ability and the expression of P53 protein and hTERT mRNA of LMP1-transfected cells were all significantly lower in asODN groups than in control groups ( P< 0 01), so were those of untransfected cells and vector-transfected cells, but in asODN groups,the proliferate ability and the expression of P53 protein of LMP1-transfected cells were still higher than those of untransfected cells and vector-transfected cells ( P< 0 01). CONCLUSION: EBV LMP1 promotes the expression of P53 protein in NPC cells. [