CXCR4在胶质瘤血管内皮细胞和ECV304细胞中的表达及其意义

Expression of CXCR4 in endothelial cells of glioma and ECV304 cells and the significance

目的 检测人胶质瘤血管内皮细胞以及体外培养的血管内皮样细胞系ECV3 0 4趋化因子受体CXCR4的表达 ,观察其配体SDF 1对血管内皮样细胞迁移的影响 ,以探讨趋化因子受体CXCR4在肿瘤血管生成中的可能作用及其机制。方法 通过免疫组化方法检测人胶质瘤血管内皮细胞CXCR4蛋白的表达 ,采用RT PCR、免疫细胞化学方法检测血管内皮样细胞系ECV3 0 4上CXCR4mRNA和CXCR4蛋白的表达 ,并采用 48孔趋化板观察SDF 1诱导体外培养血管内皮样细胞的迁移作用。结果 在胶质瘤血管内皮细胞和血管内皮样细胞系ECV3 0 4上均检测到趋化因子受体CXCR4的表达 ,体外实验显示SDF 1能诱导血管内皮样细胞发生明显的迁移。在 3 0、5 0ng ml的实验浓度范围内 ,诱导血管内皮样细胞的迁移作用呈明显量效关系。结论 胶质瘤血管内皮细胞和血管内皮样细胞系ECV3 0 4上存在CXCR4表达 ,CXCR4激活能诱导内皮细胞的迁移。

Objective To investigate the expression of CXCR4 in endothelial cells in glioma and ECV304 cells cultured in vitro and its roles in angiogenesis. Methods The expression of CXCR4 in ECV304 cells were detected by RT-PCR and immunocytochemistry. The expression of chemokine receptor CXCR4 in the endothelial cells in glioma was detected by immunohistochemistry. The migration of ECV304 induced by SDF-1β was observed by 48-well chemotaxis chamber. Results CXCR4 was expressed in the endothelial cells of glioma and cultured ECV304 cells. The chemotactic migration of ECV304 could be induced by SDF-1. SDF-1 at a concentration between 30 ng/ml and 50 ng/ml induced the chemotactic migration of ECs in a dose-dependent manner. Conclusion The results confirm that CXCR4 is expressed in the endothelial cells of glioma and the activation of CXCR4 may induce the migration of endothelial cells.