葛根素对失血性休克家兔急性肺损伤的保护作用

Protective effects of puerarin on acute lung injury in hemorrhagic shock rabbit

目的 :研究葛根素对实验性失血性休克家兔导致急性肺损伤 (ALI)的保护作用。方法 :家兔 2 4只 ,随机分为 3组。A组为假手术组 ,只进行相应的手术操作 ;B组和C组按照Wiggers改良法制作失血性休克模型 ,造成ALI,然后在 2 0min内复苏 ,C组复苏时应用葛根素。测定动脉血浆中肿瘤坏死因子 α(TNF α)含量、支气管肺泡灌洗液 (BALF)中巨噬细胞 (PAM)和中性粒细胞 (PMN)总数、BALF中蛋白含量和肺组织中超氧化物歧化酶 (SOD)含量 ;计算肺组织含水率 ;光镜下观察肺组织形态学改变。结果 :与A组比较 ,复苏后B、C组TNF α含量、BALF中的蛋白含量和PMN总数、肺组织含水率明显升高 ,SOD含量明显降低。但C组上述指标的变化程度均低于B组。光镜下见B组肺组织间质水肿、细胞浸润明显 ,与之相比C组肺组织以上改变明显减轻。结论

AIM: To study the protective effects of puerarin on acute lung injury(ALI) which was a result of experimental hemorrhagic shock in rabbits. METHODS: Twenty-four rabbits were divided into 3 groups randomly which named group A, B and C respectively. Group A was non-operation group, while hemorrhagic shock models were performed in both group B and group C according to Wiggers modified method, and resulted in ALI, then the shock rabbits were resuscitated in 20 min. The puerarin was used in group C during resuscitation. The arterial blood was taken in different time to determine the plasma tumor necrosis factor-α(TNF-α)concentration. The amount of pulmonary alveolar macrophages (PAM) and polymorphonuclear leukocyte (PMN) in bronchoalveolar lavage fluid (BALF) were counted at the end of experiment. At the same time, the protein contents in BALF and superoxide dismutase(SOD) contents in lung tissue were measured, and the water ratio of lung tissue was calculated. The histological forms of lung were examined through the light microscope. RESULTS: Compared between group A and group B, TNF-α concentration, the protein contents and the amount of PAM in BALF were increased more obviously after resuscitation. In group C the level of all indexs above was lower than that in group B. In contrast to the group A, SOD contents of lung tissue were lower in group B, with a high level of water ratio in lung tissue. In group C the SOD contents were higher than those in group B, while the water ratio was reduced. Lung tissue edema and neutrophil accumulation can be observed by microscope both in group B and group C, but the changes in the later were mollified. CONCLUSION: Puerarin has the protective effects on ALI which resulted from experimental hemorrhagic shock in rabbits.