香砂养胃丸(浓缩丸)中厚朴酚与和厚朴酚的含量测定

Determination of magnolol and honokiol in XIANGSHA YANGWEI GRANULES by RP-HPLC

目的 :建立香砂养胃丸 (浓缩丸 )中厚朴酚与和厚朴酚的含量测定方法。方法 :反相高效液相色谱法。采用ODSC1 8柱 ,流动相为甲醇 水 (70∶30 ) ,流速为 1 0mL min ,波长为 2 94nm。结果 :厚朴酚与和厚朴酚线性范围分别为 0 2 35～ 1 4 10 μg(r=0 9998)和 0 35 8～ 2 14 8μg(r=0 9997) ,平均加样回收率分别为 99 4 2 % (RSD为 0 6 6 % )和 97 15 % (RSD为 1 0 4 % )。结论 :该方法为香砂养胃丸 (浓缩丸 )

Objective:To establish a method to determine the contents of magnolol and honokiol in XIANGSHA YANGWEI GRANULES by RP-HPLC.Method:A ODS column was used.The mobile phase was methanol-water (70∶30).The flow rate was 1.0mL/min and the detective wavelength was 315nm.Results:Linear range of magnolol and honokiol were 0^235～1^410μg( r 0^9998) and 0^358～2^148μg( r 0^9997),respectively.The average recoveries were 99.42%(RSD was 0.66%) and 97.15% (RSD was 1.04%),respectively.Conclusion:The method was accurate and reliable.