苦参汤有效部位总黄酮含量测定方法研究

Study on the Method of Determination of the Contents of Total Flavonoids in the Effective Fraction of Kushen Decoction

目的 :建立苦参汤有效部位中总黄酮含量测定方法。方法 :以黄芩苷为对照品 ,采用盐酸 镁粉反应比色法测定总黄酮含量。结果 :黄芩苷在 6 971～ 34 85 7μg/mL范围内与吸光度呈良好的线性关系 ,回归方程为C =0 0 2 2 1A +0 0 0 5 3(r =0 9994 )。黄芩苷平均回收率为 10 3 5 9% ,RSD =0 74 % (n =5 )。结论 :3批样品测定结果表明 ,所建立的方法简便、准确 。

Objective To establish the method for the determination of the content of total flavonoids in the effective fraction of Kushen decoction. Methods The content of total flavonoids was determined with HCl-Mg reaction colorimetry, and selected baicalin as reference substance.Results Baicalin had a liner relationship with the absorbance in the range of 6.971～34.857μg/mL and the regression equation was as follows: C=0.0221A+0.0053(R+2 =0.9994).The average recovery for baicalin was 103.59%.RSD=0.74%( n =5).Conclusion The determination of the contents of total flavonoids of three batches of samples showed that the method was easy and accurate,and could be used to determine the contents of total flavonoids in the effective fraction of Kushen decoction.