丙酸杆菌细胞壁骨架体外对小鼠免疫机能的影响

IN VITRO EFFECTS OF PROPIONIBACTERIUM CELL WALL SKELETON(P—CWS) ON IMMUNE FUNCTIONS OF MICE

丙酸杆菌细胞壁骨架是从非致病性丙酸杆菌中提取的一种抗癌成分。其与小鼠脾脏非粘附细胞体外培养一定时间,能提高NK 细胞活性(浓度为62.5μg/ml～250 μg/m1)和ADCC 活性(浓度为31.2s～250μg/ml)。丙酸杆菌细胞壁骨架(31.25～250 μg/ml)与小鼠腹腔巨噬细胞共有48小时,能诱导产生IL—1,在低浓度(<125 μg/ml)范围内呈显量—效依赖关系。提示丙酸杆菌细胞壁骨架是一种非特异性免疫增强剂。

Propionibacterium cell skeleton(P—CWS)is an anticumor agentextracted from the non—pathogenic propionibactecium.In vitro,P—CWS(62.5—250 μg/ml)could improve the NK activity of routine nonadherent spleen cells.In a dose ranging from 31.25 to 250 μg/ml,it enhanced antibody dependent cell—mediated cytotoxicity(ADCC)of spleen cells.P—CWS could also induce perit-oneal macrophages to produce interleukin—1 when it was cultured with macro-phages for 48 hours,and it behaved in a dose-dependent manner with concentrat-ions below 125μg/ml.It is suggested that P—CWS is a nonspecific immunosti-mulant.