HPLC法同时测定氯霉素氢化可的松滴耳液中2种成分含量

Concurrent Determination of Chloramphenicol and Hydrocortisone in Chloramphenicol and Hydrocortisone Ear Drops by HPLC

目的 :建立氯霉素氢化可的松滴耳液的含量测定方法。方法 :采用高效液相色谱法 ,SpherisorbC18(4.6mm× 2 5 0mm ,5 μm) ,流动相为甲醇 0 .0 2mol·L-1KH2 PO4(用磷酸调 pH至3.0 ) (6 0∶4 0 ) ,流速 0 .9ml·min-1,检测波长为 2 4 2nm。结果 :氯霉素和氢化可的松分别在 10 0 .2～ 4 0 0 .6 μg·ml-1和 2 0 .2～ 80 .6 μg·ml-1范围内线性关系良好 ,平均回收率分别为10 0 .2 % (RSD 0 .7% )、99.8% (RSD 0 .6 % )。结论 :本方法可同时测定两种成分含量 ,操作简便 ,结果准确。

Objective: To establish a HPLC method for concurrent determination of chloramphenicol and hydrocortisone in chloramphenicol and hydrocortisone ear drops.Method: A C 18 column ( 4.6 mm×250 mm,5 μm)was used by HPLC;Mobile phase consisted of methanol 0.02 mol·L -1 polassium dihydrogen phosphate solution(pH was adjusted to 3.0 with phosphoric acid)(60∶40),the flow rate was 0.9m l·min -1 , and the detecting wavelength was at 242 nm.Result: The linear ranges of chloramphenicol and hydrocortisone were within 100.2 400.6 μg·ml -1 and 20.2 80.6 μg·ml -1 , respectively,the average recovery was 100.2% ( RSD 0.7% ) and 99.8% ( RSD = 0.6% ).Conclusion: This method can concurrently determine the two components, and being rapid and accurate.