小鼠皮肤创伤愈合过程中IL-10在不同表达部位的组化定量研究

Immunohistochemical and morphometrical study on the expression of interleukin-10 (IL-10) in different expressive parts during cutaneous wound healing in mice

目的探讨小鼠皮肤创伤愈合过程中IL-10在不同表达部位的变化与损伤时间的关系。方法应用免疫组织化学技术和形态计量法,对小鼠不同时程皮肤切创组织中IL-10不同表达部位(表皮细胞及表皮下组织的浸润细胞)的变化进行研究。结果皮肤切创前后表皮层均有阳性表达,切创后1～3h表皮细胞阳性表达水平迅速升高,24h时降至较低水平,伤后48h表达水平再次升高,96h后逐渐回复到正常水平;表皮以下阳性部位主要是浸润的单核/巨噬细胞,损伤6h后阳性细胞在真皮、皮下组织及创口肉芽组织内逐渐增多,于伤后72h达最大值(51.41±3.12)%,96～168h阳性细胞数逐渐减少(29.38±2.64)%～(5.56±4.74)%。结论皮肤创伤修复过程中IL-10在不同表达部位的变化特点与皮肤损伤时间相关,检测其表达变化可望用于损伤时间推断。

Objective To explore the expression of interleukin-10 (IL-10) in different expressive parts and its relationship with the wound age during cutaneous wound healing in mice. Methods Immunohistochemical and morphometrical analysis methods were performed on vital skin wounds. Results In normal and injury mouse skin IL-10 immunoreactivity was observed in epidermal cells. The expression of IL-10 in epidermal cells was already enhanced since 1～3 h after incision, and it returned to lower lever by 24 h, but increased again at 48 h after injury. IL-10 immunoreactivive cells under epidermis were mainly infiltrating mononuclear cells. Morphometrically, the ratio of the number of infiltrating cells was evaluated. A subsequent increase in the IL-10 positive cells was observed in the granulation tissue beyond the wound and in dermal at 6 h after incision, and the maximum ratio was (51.41±3.12)% in a 72 h-old wound. Then the positive cells gradually decreased (29.38±2.64)%～(5.56±4.74)% after incision 96～168h. Conclusions The expression characteristics of IL-10 in different expressive parts should be related to the wound age and morphometry of different expressive parts may be employed for determining the wound age.