帕金森病大鼠模型的行为学及神经元形态学评价(英文)

Evaluation on behaviours and neuron morphology of Parkinson's disease rat model

背景:由于黑质致密部体积狭小,应用6-羟基多巴胺(6-Hydroxydopamine,6-OHDA)损毁黑质多巴胺神经元(Nigraldopaminergicneuron,NDN)制作帕金森病大鼠模型的成功率较低,一般仅为30%～40%左右。目的:探讨帕金森病大鼠模型建立后的行为学及神经元形态学改变。设计:完全随机的实验研究。地点和材料:安徽省药物研究所,SD大鼠,6-OHDA,阿朴吗啡,兔抗TH血清,SR-6N大鼠脑立体定向仪等。干预:将6-OHDA立体定向注射于90只SD大鼠的左侧黑质区及黑质纹状体通路,观察大鼠行为及多巴胺神经元形态学变化。主要观察指标:旋转行为,震颤及其他异常行为,免疫组织化学观察,电镜观察。结果:经阿朴吗啡诱导共筛选出64只成功大鼠模型(占71%);免疫组化观察发现注射侧黑质区NDN较对侧明显减少,电镜观察发现其普遍存在凋亡及坏死样改变。结论:本方法是建立帕金森病大鼠模型的有效方法,在行为学和神经元形态学等方面同帕金森病人有许多相似之处。

BACKGROUND:The successful rate in the establishment of Parkinson's disease(PD) model in rat is comparatively low through destroying Nigral dopaminergic neuron (NDN) by 6-hydroxydopamine(6-OJDA) because of the small volume of pars compact a of substantia nigra(SNC),which is only about 30%to 40%. OBJECTIVE:To discuss the changes on behaviour and neuron morphology after the establishment of PD rat model. DESIGN:A complete random control study. SETTING and MATERIALS:Study was completed in the Pharmaceutical Institute of A nhui Province.Materials included SD rats,6-OHDA,apomorphine,rabbit-anti-TH se rum,SR-6N rat cerebral solid orientation apparatus,etc. INTERVENTIONS:6-OHDA was solidly and directionally injected into left SN area and corpus striatum pathway in SN in 90 SD rats to observe the changes in the b ehaviours and dopaminergic neuron morphology. MAIN OUTCOME MEASURES:Rotation behaviour,tremor and other abnormal behaviours, immunohistochemical observation,and electronic microscope observation. RESULTS:Sixty-four successful rat models(71%) were screened by the induction of apomorphine.It was found from immunohistochemical observation that NDN in th e injection side in SN significantly reduced than that of the opposite side.It w as found under electronic microscope that apoptosis and necrosis changes widely existed. CONCLUSION:This method is an effective method in the establishment of PD rat m odel,which has many similar aspects in behaviour and neuron morphology compared with PD patients.