电针对大鼠脑内前速激肽原AmRNA表达的影响(英文)

Influence of electroacupuncture on the expression of preprotachykinin A mRNA in the brain of rat

背景:速激肽广泛地分布于中枢神经系统中,针刺的信息要经过许多神经递质或调制物质的转递才能产生镇痛效果,有关针刺引起脑内速激肽变化的研究少见报道。目的:观察电针对大鼠脑内前速激肽原(preprotachykinin,PPT)AmRNA表达的影响。设计:完全随机设计,对照实验研究。地点和材料:西安交通大学医学院生理教研室,用健康Sprague-Dawley大鼠30只进行实验。大鼠体质量220～250g,由西安交通大学基础医学院动物中心提供,雌雄不拘,清洁级。干预:在电针大鼠下肢“足三里”穴位后,用原位杂交的方法观察电针穴位后24h大鼠脑内PPTAmRNA表达。主要观察指标:观察尾壳核、杏仁核、下丘脑室旁核、下丘脑前区及导水管周围灰质PPTAmRNA表达的变化。结果:电针组尾壳核、杏仁核、下丘脑室旁核、下丘脑前区及导水管周围灰质PPTAmRNA表达阳性细胞数较对照组高(分别是11.20±2.56,10.63±2.57,6.52±1.12,8.16±1.82,7.351±1.59),刺激强度为5mA的强电针组上述部位PPTAmRNA表达(分别是16.56±3.31,15.66±3.03,10.20±2.88,12.13±2.86,12.16±2.44)比刺激强度为1mA的弱电针组(分别是13.38±2.48,12.83±2.86,8.33±1.76,10.00±2.58,10.12±2.37)高。结论:电针能引起大鼠脑内一些部位PPTAmRNA表达的增高,速激肽的合成增加,因而?

BACKGROUND:Tachykinins are extensively distributed in central nervous system(C NS).Acupuncture signals are mediated by a whole batch of neurotransmitters or ne uromodulators to manifest an analgesic effect.There were few studies on the infl uence of electroacupuncture(EA) on the expression of preprotachykinin(PPT) A mRN A in the brain. OBJECTIVE:To study the influence of EA on the expression of PPT A mRNA in the brain of rat. DESIGN:A completely randomized controlled study. SETTING and PARTICIPANTS:Thirty healthy adult Sprague-Dawley rats weighing 22 0-250 g each were used in this study in the Department of Physiology of the Med ical College of Xi'an Jiaotong University.Animals of either sex and of clearing grade were provided by the animal center of Basic Medical College of Xi'an Jiaot ong University. INTERVENTIONS:The expression of PPTAmRNA in the brain of rat was examined with the method of hybridizable histochemistry in situ 24 hours after electroacupunc turing the “zusanli”acupoint(ST 36). MAIN OUTCOME MEASURES:The expression of PPTAmRNA in amygdaloid nucleus(Amy),ca udate putumen(CPu),anterior hypothalamic areas(AH),paraventricular hypothalamic nucleus(Pa) and periaqueducted grey(PAG). RESULTS:The number of positive cells expressing PPTAmRNA in the group of EA in Amy,CPu,AH,Pa and PAG was larger than those of the control group(11.20±2.56,1 0.63±2.57,6.52±1.12,8.16±1.82,7.351±1.59,respectively).The number of neur ons expressing PPTAmRNA in these areas in the group of 5 mA EA(16.56±3.31,15. 66±3.03,10.20±2.88,12.13±2.86,12.16±2.44,respectively)were larger than t hose of the group of 1 mA EA(13.38±2.48,12.83±2.86,8.33±1.76,10.00±2.58 ,10.12±2.37,respectively). CONCLUSION:EA could increase the expression of PPTAmRNA and the synthesis of t achykinins in some areas of the brain of rat,and thereby may reinforce the regul ation influence on many functions of the body.