HBV DNA PreC/C和BCP片段基因多态性及临床意义

Research for Gene Mutation in the Areas of PreC/C and BCP of HBV DNA and Clinical Application

目的 :分析乙肝患者 HBV DNA前 C/ C区和基本核心启动子区部分 DNA片断突变。方法 :PCR扩增HBV DNA nt1735～ 196 5片段 ,将产物进行 HBV DNA测序。结果 :在 6 8例乙肝患者中 ,突变阳性率 4 8.5 % ,点突变16 8个 ,频率前 10位的是 nt176 4、176 2、1799、176 6、1896、175 4、1899、176 8、1814及 1913,还检出鲜见报道的 192 3、192 2、190 7等点突变。慢性肝炎 5 4例和肝炎肝硬化 10例 HBV DNA nt1896、176 4、176 2点突变阳性数分别为 9、19、19和 3、19、19,两者差异有极显著性 (P<0 .0 1)。结论 :前 C/ C区与基本 C区启动子区基因突变可能与肝实质纤维化相关 ,HBV DNA突变发生率较高 ,且位点众多 。

Objective:to investigate the gene mutation in the areas of precore/core(PreC/C) and basal core promotor(BCP) of HBV DNA with gene sequencing.Method:The nt1735～1965 segment of HBV DNA were amplified .Then the PCR production was sequenced.Results:The mutative frequence was 48.5 percent in 68 patients with hepatitis B,and the site mutation of 168 was deteced as well.The top ten mutative site was nt1799,1766,1896,1754,1899,1768,1814 and 1913 respectively.The site mutative at nt1923,1922 and 1907 was found,which is rare.The preliminary clinical experiment showed the statistical difference between the patients with chronic hepatitis B and the patients with post hepatitis cirrhosis.Conclusions:The assay suggested that the mutation in the areas preC/C and BCP of HBV DNA be relevant to liver fibrosis potentially;there is a higher mutative frequence in hepatitis B virus,and gene sequencing is a useful guidance in research for gene chip and in clinical application.