五味活血化瘀中药对SPC-A-1细胞凋亡影响的研究

The Effects of Five HuoXueHuaYu Chinese Medicine on SPC-A-1 Cell

目的 :观察活血化瘀中药对癌细胞生长和凋亡的影响。方法 :运用药物血清学方法 ,采用MTT法 ,观察露蜂房、赤芍、铁树叶、石见穿、泽兰叶 5味常用活血化瘀抗癌中药对SPC -A -1肺腺癌细胞株生长的抑制率 ,并采用流式细胞术对SPC -A -1肺腺癌细胞株进行Annexin -V凋亡检测 ,探讨 5味活血化瘀中药在肿瘤生长、凋亡中的作用。结果 :MTT法示铁树叶对SPC -A -1细胞培养早期RI达 2 6% ,其余 4味中药晚期RI分别达 3 1.9%、3 7.2 6%、3 2 .12 %、40 .8%。其余 4味则显示出作用时间越长抑制作用越明显的特点。Annexin -V凋亡检测 :5味药物的LR分别为 8.5 3 %、8.3 6%、13 .2 2 %、7.87%、9.73 % ;UR分别为69.47%、70 .72 %、67.62 %、75 .5 9%、66.72 %。结论 :5味活血化瘀对SPC -A -1人肺腺癌细胞均有一定的抑制和促凋亡作用 ,除铁树叶有一定促细胞早期凋亡的作用外 ,其余 4味均主要促进癌细胞的晚期凋亡。

Objective: To study the effects of five Chinese medicine (Nidus vespae, Radix Paeoniae Rubra, Folium Cycadis Revoluta, Herba Salviae Chinensis, Herba Lycopi) on grow and apoptosis of SPC-A-1 cell. Methods: MTT assay and FCM.Results: MTT assay show that the early RI of Folium Cycadis Revoluta is 26%, the late RI of the other medicines are 31.9%,37.26%,32.12%,40.8%. Annexin-V test show that the LR are 8.53%, 8.36%, 13.22%, 7.87%,9.73% and the UR are 69.47%,70.72%,67.62%,75.59%,66.72% seperaterly. Conclusion:Chinese medicine inhibited SPC-A-1 cell .The inhibited effect of Folium Cycadis Revoluta was in early period and the others were in time-dependent manner. In Apoptosis test by FCM Folium Cycadis Revoluta bring on early apoptosis.