同种和异种混合淋巴细胞反应及分类混合淋巴细胞反应的比较

Comparison of mixed lymphocytic reactions to both xenogenic murine and allogenic human lymphocytes

目的:对比体外人外周血淋巴细胞对于异种小鼠细胞和同种异体细胞混合淋巴细胞反应. 方法:建立异种-同种混合淋巴细胞反应模型,进行分类细胞反应试验. 结果:人外周血淋巴细胞对于异种小鼠细胞的反应明显低于对于同种异体细胞的反应(P<0.05).细胞分类研究显示:在异种混合淋巴细胞反应中,主要是CD4+T细胞通过间接途径受小鼠抗原刺激而增生;在同种混合淋巴细胞反应中,主要是CD4+T细胞通过直接途径受同种异体抗原刺激而增生.在两种混合淋巴细胞反应中,CD8+T细胞也参与. 结论:异种混合淋巴细胞反应弱于同种混合淋巴细胞反应, 异种细胞只通过间接途径刺激T细胞.同种和异种混合淋巴细胞反应均需要抗原提呈细胞参与,CD4+T淋巴细胞是反应的主要细胞.

AIM: To compare the mixed lymphocyte reactions (MLR) of human lymphocytes response to xenogenic murine lymphocytes and the allogenic human lymphocytes in vitro. METHODS: Xeno-and allo- MLRs were set up and cellular classification study of xeno- and allo- MLRs were performed. RESULTS: Human T cells response to xeno-cells was weaker than to allo-cells (P <0.05). Cellular classification study indicted that it was mainly CD4+ T cell involved in both allo- and xeno-MLRs, the former through direct and indirect pathways and the latter through indirect pathway. In addition, CD8+ T cell was involved in the reaction as well. CONCLUSION: Xeno-MLR is weaker than allo-MLR. Xenogenic cells can only stimulate T cells through indirect pathway. Both MLRs share similar requirements for APCs, and human CD4 T cells are the major response cells.