聚β-羟基丁酸酯作为肾上腺细胞载体的实验研究

Transplantation of Adrenocortical Cells in Allorat Using Porous PHB as Cell Carrier

为了探索聚 β-羟基丁酸酯 (PHB)作为肾上腺细胞移植载体的可行性。将肾上腺细胞与 PHB共培养 ,观察 PHB对体外培养的肾上腺细胞增殖、分泌功能的影响。将肾上腺细胞植入到受鼠体内 ,观察受鼠体内激素水平的动态变化及局部组织学变化。肾上腺细胞可以在 PHB上生长 ,PHB对体外培养的肾上腺细胞的增殖、分泌功能无影响。实验组大鼠体内激素水平比单纯双侧肾上腺切除组的激素水平要高 ,两者差别有显著性 (P<0 .0 5 )。PHB对肾上腺细胞的生长、增殖、分泌功能无影响。PHB在体内逐步降解。PHB作为细胞载体应用于肾上腺细胞移植具有可行性。

This experiment was designed to investigate the feasibility of transplanatation of using porous PHB as cell carrier for the transplanatation of adrenocortical cells. Adrenocortical cells from rat adrenal gland were separated and cultured in vitro. The effect of PHB no the proliferation and secretory function of adrenocortical cells were evaluated by MTT and RIA methods. Then adrenocortical cells were seeded into porous PHB. After the cells were cultured in vitro for about seven days,they were implanted into the rats having undergone bilateral adrenalectomy. The changes of blood corticosterone and aldosterone and the local histological changes in these rats were observed. Adrenocortical cells were able to grow and survival on PHB. No effect on the proliferation and secretory function of adrenocortical cells were observed. Most bilateral adrenalectomized rats bearing the transplanted adrenocortical cells within PHB(study group)survived longer than did the adrenalectomized rats in control group. The blood corticosterone level and aldosterone level of study group were higher than those of control group.It was found that PHB has no effects on the survival,proliferation and secretory function of adrenocortical cells. Adrenocortical cells within PHB can survivel a period of time and can secrete corticosterone and aldosterone which can meet the needs of the adrenalectomized rats. PHB can degrade slowly in vivo. It is feasible to perform transplantation of adrenocortical cells using porous PHB as cell carrier.