拉米夫定治疗慢性乙型肝炎患者血清及PBMC内HBV DNA的动态变化

Dynamic Changes of Hepatitis B Virus DNA in Serum and in Peripheral Mononuclear Cells in the Patients with Lamivudine Therapy

目的 :研究拉米夫定对慢性乙型肝炎 (慢乙肝 )患者血清及PBMC内HBVDNA的抑制作用。方法 :应用荧光定量PCR方法动态观察 72例拉米夫定治疗组和对照组患者血清及PBMC内HBVDNA的含量变化。结果 :治疗组血清HBVDNA在治疗 6周时 ( 2～ 8周 ) 2 1例转阴 ,PBMC中HBVDNA在治疗 16周 ( 8～ 2 4周 )时 17例转阴。结论 :拉米夫定对慢乙肝患者血清HBVDNA有较强的抑制作用 ,起效快 ,同时 ,随着治疗时间的延长 ,对PBMC内HBVDNA也有一定的抑制作用 。

Objective:To study the dynamic changes of hepatitis B virus DNA in serum and in peripheral mononuclear cells in the patients with Lamivudine therapy.Methods:Seventy-two patients with chrnic hepatitis B virus (HBV) infection were investigated for HBV DNA both in serum and in peripheral blood mononuclear cells(PBMCs) by fluorescence quantitative polymerase chair reation(PCR),during and after Lamivudine treatment.Results:HBV DNA both in serum and in PBMCs, become negative in 21 cases and 17 cases respectively during Lamivudine treatment. The average conversion period of HBVDNA was 6w (2-8w) in serum and 16w (8-24w) in PBMCs. Conclusion:Lamivudine has remarked inhibitory effect on HBV DNA both in serum and in PBMCs. The inhibitoty effect on HBV DNA in PBMCs was sloowe than in serum.