纳洛酮对心肺复苏犬脑组织S100蛋白表达的影响

Influence of expression of brain S100 protein induced by cardiopulmonary resuscitation of canines with naloxone

目的 通过检测心搏骤停犬复苏后及给予纳洛酮干预后脑组织中S1 0 0蛋白表达情况 ,了解纳洛酮对脑复苏的影响。方法 1 8只健康杂种犬 ,随机分成 3组 ,每组 6只 ,予体外电击诱发室颤 ,对照组 :心搏停后予标准心肺复苏术 ;实验组 :心搏骤停后予标准心肺复苏术 +纳洛酮 ;空白组 :不诱发室颤 ,于复苏后 6h取脑海马组织行脑形态学检查 ,及S1 0 0蛋白表达的测定。结果 实验组S1 0 0蛋白表达明显低于对照组 (P <0 0 1 ) ,实验组脑组织的病理损害低于对照组。结论 使用纳洛酮后心肺复苏犬脑组织的病理损害有所减轻 ,脑组织S1 0 0蛋白的生成也显著减少 ,纳洛酮可能通过减少S1 0

Objective In order to study the effect of Naloxone in cerebral resuscitation, we examine the expression of S 100 protein of cerebral tissue in only restore of spontaneous circulation (ROSC) and with Naloxone after cardiac arrest.Methods 18 canines were randomly divided into CPR group, Naloxone group and blank group, n =6.Ventricular fibrillation (VF) was induced in CPR group and Naloxone group by the external electricity. The hippocampus tissue was taken out after the 6 hour procedure and observed the morphologic change and measured the expression of S 100 protein.Results The expression of S 100 in Naloxone group was lower than that in CPR group( P <0 01). Compared with the CPR group, the pathological damage was mild.Conclusion Naloxone treatment could decreased the pathological damage and expression of S 100 protein in cerebral tissue of post resuscitated canines. Naloxone may be improved the reperfusion injury of post resuscitation through decreased expression of S 100 protein.