IL-12和IL-18的体外联合孵育协同增强P0_(180-199)T/Th1细胞增殖和分化的实验研究

IL-12 and IL-18 synergically potentiate the proliferation and differentiation of the T cell lines specific for myelin-specific P0 protein peptides P0_(180-199)

目的 研究 IL- 12和 IL- 18是否能够协同增强 P0 1 80 - 1 99T/ Th1淋巴细胞的增殖和分化。方法 用 IL-12和 /或 IL- 18体外孵育的 P0 1 80 - 1 99T细胞过继免疫正常动物 ,引发 AT- EAN后 ,用流式细胞仪检测单独组和联合组的脾 T淋巴细胞的增殖 ,EL ISA法检测单独组和联合组的细胞培养上清和血清中 Th1和 Th2细胞因子的变化。结果 与 IL- 12组或 IL- 18组比较 ,IL- 12和 IL- 18的联合孵育使 P0 1 80 - 1 99T细胞的 CD4 +T/ Th1分化能力增强 ,表现为脾细胞的 CD4 +/ CD8+的比值显著上调 ,细胞培养上清和 AT- EAN血清中 IFN-γ的显著上调 ,具有显著性差异。结论 IL - 12和 IL - 18能够协同增强 P0 1 80 - 1 99T/

Objective To study the synergetic potentiation of IL-12 and IL-18 to the proliferation and differentiation of the specific T cell lines to P0 180-199 .Method To coculture the P0 180-199 specific Tcell lines with IL-12 and/or IL-18,adoptive transfer the cells to induce EAN,access the splenic proliferation by FASC and compare the cytokines level in the cell culture supernatants and AT-EAN sera by ELISA.Results Splenic CD 4 + lymphocytes were upregulated and CD 4 +/CD 8 + were increased statisticallyonly in presence of both IL-12 and IL-18 by flow cytometry.IL-12 induced P0 180-199 Th1 cell differentiation through IFN-γ,while IL-18 induced P0 180-199 Th1 cell differentiation through TNF-α,P0 180-199 T/Th1 cells were wellproliferated and activated in the presence of IL-12 and IL-18 compared to the IL-12 orIL-18 group.It were demonstrated by the high level of IFN-γ in the cell culture supernatants and AT-EAN sera by ELISA.Conclusion IL-12 andIL-18 have a synergic potentiation in P0 180-199 T/Th1 cell proliferationand differentiation.