趋化素样因子2 mRNA在心肌肥厚大鼠心肌中的表达

Expression of chemokine-like factor 2(CKLF2) mRNA in rat myocardium with hypertrophy

目的 :检测趋化素样因子 2 (chemokine likefactor 2 ,CKLF2 )mRNA在心肌肥厚大鼠心肌中的表达。方法 :实验组以手术缩窄大鼠腹主动脉致心肌肥厚 ,对照为假手术组 ;应用竞争PCR方法检测两组左心室CKLF2mRNA表达差异。结果 :与假手术组相比 ,手术组术后早期 (第 1 7天 )心肌中CKLF2mRNA表达增高 ,第 4 5天时降到假手术组水平。结论

Objective: To investigate the expression of CKLF2 mRNA in the rat myocardium with hypertrophy. Methods: Rat abdominal aorta was constricted by operation to make myocardium hypertrophy in the hypertrophy gronp and the control group was subjected to the sham operation. The CKLF2 mRNA expressions were tested by competitive polymerase chain reaction (CPCR). Results: Compared to the control group, the expression of CKLF2 mRNA of hypertrophy group significantly increased on the 17th day after operation, and decreased to the control level on the 45th day. Conclusion: The expression of rCKLF2 mRNA in rat myocardium with hypertrophy significantly increased in early days of hypertrophy.