褪黑激素对耐力训练小鼠一次力竭运动后肝、肾细胞超微结构的影响

Influence of Melatonin on Ultrastructure of Liver Cell and Kidney Distal Convoluted Tubule Cell in Endurance-trained Mice after Exhaustive Exercise

目的 :通过运动训练动物模型探讨MT对运动状态下机体的影响。方法 :以昆明种雄性小鼠为实验对象 ,递增强度耐力训练后进行一次力竭性游泳 ,分别取小鼠肝脏、肾脏 ,透射电子显微镜观察其组织细胞的超微结构。结果 :连续 5 1d服用MT后 ,安静对照组小鼠肝与肾细胞超微结构无明显改变。耐力训练的同时补充MT可使小鼠力竭游泳时间显著延长 (P <0 0 1) ;力竭性运动对肝、肾超微结构有明显破坏作用 ,服用MT小鼠的肝、肾超微结构破坏较轻。结论 :MT一定程度上可对抗力竭性运动所致的超微结构的破坏 ,并有部分促进恢复作用。MT作为机体运动时的抗氧化剂或体能恢复剂值得进一步关注。

Purpose:To introduce MT into sports medicine, the effect of MT on animal experimental model during exercise was studied. Methods: The experimental animals were male Kunming mice. The model of progressive intense endurance training was developed. Ultrastructure of liver and kidney cell after an exhaustive exercise were investigated by transmission electron microscope. Results: No obvious change was found in ultrastructure of liver and kidney after 51 days MT administration, but the exhausive swimming time was significant prolonged in endurance-trained mice(P<0.01). Exhaustive exercise considerably damaged the ultrastructure of liver and kidney, and MT could reduce the damage in some extent. Conclusion: MT had a protective effect on cellular structure during exhaustive exercise, and might be used as antioxidant for athletes in the future, but needs further research.