X射线照射诱导小鼠junB基因的表达

Expression of junB gene induced by X-rays in mice

目的 探讨整体照射和不同剂量X射线离体照射后小鼠脾细胞和白血病细胞junB基因的表达。方法 3GyX射线小鼠全身照射及不同剂量X射线照射离体脾细胞和白血病细胞 ,提取RNA ,Northern杂交 ,定量分析junBmRNA。结果 3GyX射线全身照射后 ,小鼠脾细胞junB基因增强明显并迅速 ,C3H He小鼠 30min达高峰 ,BALB c小鼠 6 0min达高峰 ;不同剂量X射线照射离体脾细胞和白血病细胞后 ,junB基因也迅速被诱导 ,30～ 6 0min达峰值 ,2 4 0min逐渐降回假照组水平。结论 junB基因是一个辐射敏感基因 ,照射后立即表达 ,有可能在信号传递中起重要作用。

Objective To explore junB gene expression in spleen cells after whole body irradiation and in cultured spleen cells as well as leukemia cells irradiated with different doses of X-rays. Methods Spleen cells were pooled with MEM-α medium after 3 Gy X-ray whole body exposure,and normally cultured spleen cells and leukemia cells were irradiated with different doses of X-rays. Total RNA was extracted at intervals,Northern blot hybridization was used to present the expression of junB mRNA,and the RNA ratio of junB/β-actin/control was calculated to show the level of junB mRNA. Results After mice were exposed whole-bodily to 3 Gy X-rays,appearance of junB expression in spleen cells was remarkable and rapid,reaching its peak at 30 min for C3H/He mice,60 min for BALB/c mice. For normally cultured spleen cells and leukemia cells followed by different dose irradiation,junB mRNA increased immediately after irradiation,reaching its peak at 30 min or 60 min,returned gradually to the normal level within 240 min. Conclusion junB gene is sensitive to radiation,for it is induced immediately after irradiation. junB gene may play an important role in process of message transduction.