焦炉工外周血淋巴细胞遗传物质损伤水平的研究

Genetic damage in peripheral blood lymphocyte of coke oven workers

目的 评价焦炉作业环境接触多环芳烃对焦炉工外周血淋巴细胞遗传物质的损伤。方法 应用彗星试验和外周血淋巴细胞胞质分裂阻滞微核检测法 ,评价 13 7名焦炉工和 50名非职业多环芳烃暴露对照人群外周血淋巴细胞DNA和染色体损伤水平 ;测定其尿中 1 羟基芘水平 ,评价个体多环芳烃暴露内剂量 ;收集个人职业史、年龄、性别、吸烟和饮酒状况等信息。结果 焦炉工尿中 1 羟基芘水平为 (5.76± 1.0 4) μmol/molCr ,明显高于对照组 [(0 .70± 0 .3 2 ) μmol/molCr]。焦炉工外周血淋巴细胞微核率和彗星尾矩分别为 8.0‰ (0 .0‰～ 3 0 .0‰ )和 2 .0 9(0 .3 1～ 75.41) ,均高于对照组 [3 .5‰ (0 .0‰～13 .0‰ )、1.0 5(0 .11～ 6.63 ) ] ,差异有显著性 (P <0 .0 5)。对照组中 ,吸烟个体彗星尾矩为 1.44(0 .2 3～6.63 ) ,高于不吸烟个体 [0 .81(0 .11～ 3 .47) ] ,差异有显著性 (P <0 .0 5)。按焦炉作业工龄将 13 7名焦炉工分为 0 .5～、16.0～和 2 2 .0～ (年 ) 3组 ,校正了年龄、性别、吸烟、饮酒和尿中 1 羟基芘水平后 ,焦炉工外周血淋巴细胞彗星尾矩分别为 1.3 4 (0 .3 1～ 3 7.84)、2 .3 2 (0 .49～ 52 .97)和 3 .2 0 (0 .45～ 75.41) ,有随焦炉作业工龄增加而增加的趋势。

ObjectiveTo investigate DNA and chromosome damage in peripheral blood lymphocyte of coke oven workers exposed to polycycli c aromatic hydrocarbons(PAHs). MethodsOne hundred and th irty-seven coke oven workers and 50 controls without occupational PAHs exposure were investigated.Comet assay and cytokinesis-block micronucleus(CBMN) detecti on were used to evaluate DNA and chromosomal damage levels in peripheral blood l ymphocyte.Urinary 1-hydroxypyrene level was used to assess the personal interna l PAHs exposure dose.Personal information including occupational history,age,sex ,smoking and drinking status was collected by questionnaire. Results Urinary 1-hydroxypyrene level in coke oven workers[(5.76±1.04)μ mol/mol Cr] was significantly higher than that in controls[(0.70±0.32)μmo l/mol Cr].The rate of CBMN and comet tail moment of lymphocyte in coke oven wor kers[8.0‰(0.0‰～30.0‰) and 2.09(0.31～75.41),respectively] were higher than those in controls[3.5‰(0.0‰～13.0‰) and 1.05(0.11～6.63), P <0.05].In co ntrols,the comet moment in smokers was significantly higher than that in non-sm okers [1.44(0.23～6.63) vs 0.81(0.11～3.47), P < 0.05] .According to t he length of work,137 coke oven workers were classified into 3 groups i.e.0.5 yr s～,16.0 yrs～ and 22.0 yrs～ group,and the comet moments were 1.34(0.31～37.84) ,2.32(0.49～52.97) and 3.20 (0.45 ～75.41) respectively after adjusting the ag e,sex,smoking,drinking and level of urinary 1-hydroxy-pyrene.There was a risin g tendency along with the increase in length of work. Conclusion Under present PAHs exposure levels,both comet assay and Cytokinesis -block micronucleus test could detect PAHs-induced genotoxicity in coke oven w orkers,and comet assay is more suitable to assess the cumulative damage effect o n DNA.