转化生长因子β_1在个旧矿工肺癌和其他地区肺癌中表达的比较研究

Comparison of TGF-β_1expression of lung cancer in Gej iu miners with that in non-miners in other regions

目的 研究转化生长因子 β1(TGF β1)在矿工肺癌 (个旧 )和非矿工肺癌中的表达差异 ,探索个旧矿工肺癌高发的原因。方法 以个旧矿工肺癌 3 0例、其他地区非矿工肺癌 3 0例为研究对象 ,应用免疫组化和原位分子杂交方法检测其肺癌组织TGF β1蛋白水平及其mRNA的表达 ,并应用图像分析系统对每组各 10例进行了定量分析。结果 个旧矿工肺癌、非矿工肺癌中TGF β1表达的阳性率分别为75.3 9%和 44.78% ,TGF β1mRNA阳性率分别为 63 .96%和 3 4 .0 7% ,两组之间差异均有高度显著性 (P <0 .0 1)。结论 个旧矿工肺癌中TGF β1的表达明显高于非矿工肺癌 ,个旧矿工肺癌的发病机制与非矿工肺癌可能有所不同。TGF

ObjectiveTo investigate the different exp ression of transforming growth factor β 1(TGF-β 1) in lung cancer specimens of Gejiu miners, and non-miners in other regions. MethodsThirty specimens of Gejiu miners' lung cancer and 30 specimens of non-miner s' were observed in this experiment.The expression of TGF-β 1 protein and TGF -β 1 mRNA were detected by the methods of immunohistochemistry and in situ hy bridization.The results were quantitatively analyzed using image analysis system . ResultsThe positive rate of TGF-β 1 protein express ion in Gejiu miners and non-miners was 75.39%and 44.78% respectively,and the po sitive rate of TGF-β 1 mRNA was 63.96% and 34.07% respectively.There were sig nificant differences between the two groups( P < 0.01) . Conclusi onThe expression of TGF-β 1 in lung cancer of Gejiu miners was s ignificantly higher than that of non-miners.The pathogenesis of lung cancer may be different between Gejiu miner and non-miners.High expression of TGF-β 1 may be one of the reasons of high incidence of lung cancer in Gejiu miners.