HIF-1α和iNOS在大肠癌组织血管形成中的作用

Significance of HIF-1α and iNOS in the Angiogenesis in Colorectal Cancer

目的 探讨缺氧诱导因子 1α(HIF 1α)和诱导型一氧化氮合酶 (iNOS)在大肠癌血管形成中的作用。方法 采用免疫组化S P法 ,检测 62例大肠癌组织及 10例癌旁正常组织中HIF 1α和iNOS蛋白的表达 ,并用CD3 4标记血管内皮细胞 ,计算微血管密度 (MVD )。结果 62例大肠癌组织中 ,HIF 1α、iNOS蛋白阳性表达率分别为 43 .5 % ( 2 7/62 ) ,5 6.5 % ( 3 5 /62 ) ,MVD平均值为 2 8.3± 17.5。HIF 1α、iNOS蛋白阳性表达率及MVD与癌组织Dukes分期显著相关 (P均 <0 .0 5 )。HIF 1α与iNOS表达一致符合率为 5 1.6% ( 3 2 /62 ) ,两者表达显著相关 (P <0 .0 5 )。HIF 1α、iNOS均阳性的大肠癌组织MVD高于两者均为阴性者 ,有显著性差异 (P <0 .0 5 )。结论 大肠癌组织存在HIF 1α蛋白的过表达 ,HIF 1α诱导iNOS蛋白表达 ,协同促进肿瘤新血管形成。HIF 1α。

Objective To study the significance of the expression of HIF-1α and iNOS in the angiogenesis in colorectal cancer.Methods HIF-1α and iNOS protein expression were detected by immunohistochemical method in 62 cases of Colorectal Cancer and 10 cases of tumor surrounding normal mucosa.CD34 immunostain was used to measure the microvascular density(MVD).Results The Positive expression of HIF-1α and iNOS protein were 43.5%(27/62) and 56.7%(35/62) in colorectal cancer.The medium MVD was 28.3±17.5.The expression of HIF-1α and iNOS protein and the MVD were significantly correlated with Dukes stage of the tumor(P<0.05).The coincidence rate of the expression of HIF-1α and iNOS was 51.61%(32/62),showing a close relation between them.MVD was much higher in tumor with HIF-1α(+)/iNOS(+) than in tumor with HIF-1α(-)/iNOS(-)(P< 0.01).Conclusion HIF-1α protein is over-expressed in colorectal cancer.The expression of iNOS protein is induced by HIF-1αprotein and they have a synergistic effect in the neovascularization of the tumor.The expression of HIF-1α and iNOS and the MVD can be as indicators for the metastasis in colorectal cancer.