血管内皮生长因子及受体Flt-1在膀胱癌中的表达及意义

Expression of VEGF and the receptor in bladder carcinoma and clinical significance

目的探讨血管内皮生长因子 (VEGF)及其受体Flt 1在膀胱癌血管生成中的作用及与膀胱癌分化程度的关系。方法采用免疫组化S P法对 4 0例膀胱癌及 5例正常膀胱组织中VEGF、Flt 1蛋白进行检测。结果VEGF及其受体Flt 1主要分布在膀胱癌组织血管内皮细胞和癌细胞胞质内 ,VEGF及Flt 1在G3(低分化 )膀胱癌血管内皮细胞和癌细胞中的表达高于G2 (中分化 )和G1(高分化 )并且明显高于正常组织 (P <0 0 1)。结论VEGF和其受体Flt 1与膀胱肿瘤的分级和分期密切相关 ,可以作为判断膀胱癌生长和浸润的指标之一。

Objective The relationship between the expression of vascular endothelial cell growth factor (VEGF), the receptor Flt-1, the pathological types and clinical stages in bladder cancers were studied. Methods The expression of the receptor Flt-1 and VEGF in the 40 cases bladder cancer and 5 cases normal bladder tissues were assayed by immunohistochemical of S -P with the VEGF and the Flt-1 polyclonal antibody. Results The VEGF and receptor Flt-1 were widely expressed in the cytoplasm of endothelial cells and tumor cells of endometrial carcinoma. The level of VEGF and Flt-1 proteins in the endothelial cells and bladder cancer cells of grade II and III tumor tissues were higher than that in grade I and normal bladder tissues (P<0.01).Conclusion Expression of VEGF and Flt-1 related to the pathological types and clinical stages of bladder cancers. They could be used as the useful indexes reflecting the biological behavior and might be considered to be a reference indicator for judging prognosis and selecting clinical therapy of bladder carcinoma.