大鼠脑挫伤后脑组织COX-1/COX-2的表达

An immunohistochemical study on COX-1 and COX-2 following traumatic brain Injury in rats

目的 观察脑损伤后COX 1和COX 2蛋白表达及其时序性变化 ,探讨脑损伤的分子机制及法医学脑损伤时间推断。方法 以自由落体撞击雄性SD大鼠右顶叶制备脑挫伤模型 ,用免疫组织化学SP法处理脑组织检材 ,观察不同时间 ( 1、 3、 5、 7、 14d)脑组织COX 1/COX 2蛋白的表达情况。结果 正常及手术对照组大鼠 ,脑组织内有低水平的COX 1/COX 2表达 ;脑挫伤后 1～ 5d ,大鼠脑组织内COX 1表达逐渐增加 ,14d时仍维持在高表达水平 ;脑挫伤后 1～ 3d ,大鼠脑组织皮质内COX 2表达逐渐增加 ,1d时海马COX 2表达达高峰。结论 脑挫伤后可诱导COX 1/COX 2蛋白在脑内表达 ,并呈现出时序性变化。

Objective The time-dependent changes of COX-1 and COX-2 following the experimental brain contusion were studied for the purpose of extrapolation of the molecular mechanism and timing of brain contusion.Methods Male SD rats were divided into normal control,sham-operated control and contusion groups.The animal model of cerebral contusion was established by impact to the parietal lobe with a free fall weight.The time-dependent changes of COX-1 and COX-2 were detected at 1d,3d,5d,7d,14d post-injury by immunohistochemical SP method.Results In comparison with the control,COX-1 and COX-2 were faintly expressed in the brain of the control groups.Expression of COX-1 was gradually elevated in the cortex of the brain from 1d to 5d after contusion,which was sustained at a high level up to 14d postinjury.Expression of COX-2 was gradually increased in the cortex of the brain from 1d to 3d after injury,which peaked in the hippocampus at 1d after contusion.Conclusion It is suggested that brain contusion may induce the expressions of the COX-1 and COX-2,and the time-dependent changes of COX-1 and COX-2 may be applicable to the wound age estimation of cerebral contusion in forensic practice.