摘要
目的 探讨动脉球囊损伤后 ,左旋精氨酸抑制内膜增生的机制。方法 36只健康家兔随机分成球囊损伤组(A组 ,n =12 )、球囊损伤 +L -Arg组 (B组 ,n =12 )、假手术组 (S组 ,n =12 ) ;每组再随机分成术后 7天处死和术后 2 8天处死两个亚组 (n =6 )。A、B组行右髂动脉球囊损伤术。B组从术前 3天至处死时经静脉注射L -Arg 95 0mg·Kg-1·d-1。组织切片行PCNA免疫组化染色 ,行形态学观察及形态计量分析。结果 A、B、S组皆有PCNA表达 ,A7组较B7、S组PCNA阳性细胞数、阳性百分率、增殖指数显著增高 (P <0 .0 5 )。结论 应用L -Arg可抑制动脉球囊损伤后内膜的增生 ,其作用的机制可能与L -Arg能抑制动脉壁细胞PCNA表达有关。
Objective To investigate the mechanism of L-Argine on intimal proliferation after percutaneous balloon artery injury.Methods 36 rabbits were divided randomly into three groups: balloon de-endothelization group(Group A, n=12), balloon de-endothelization plus L-Argine group(Group B,n=12), sham operation group(Group S, n=12).Each group was redivided randomly into two subgroups(n=6) according to the time animal to be killed. The right iliac arteries of all rabbits in Group A and Group B were damaged with percutaneous balloon dilation. The animals in Group B were injected transvenously with L-Argine( 950mg·Kg-1/d) from 3 days before operation till the time they were killed. Pathomorphological examination and proliferating cell nuclear antigen immunohistochemistry assay were performed. Results PCNA positive cells and PCNA positive percentage in Group A 7 were significantly more than that in Group B 7 and in Group S (P<0.05). The cell proliferation index in Group A 7 was significantly higher than that in Group B 7 and Group S (P<0.05). Conclusions L-Argine can inhibit effectively intimal proliferation after balloon artery injury. The mechanism is that L-Argine can inhibit PCNA expression of arterial wall cells.
