摘要
目的检测胰腺癌中雌激素受体α(ERα)不同变异体ERC4、ERD3、ERD5mRNA的表达。方法采用免疫组化和RT-PCR检测技术,对50例胰腺癌和20例乳腺癌患者ERα的表达以及变异体ERC4、ERD3、ERD5mRNA的表达进行检测。结果胰腺癌ERα蛋白的阳性率为32％。ERD3mRNA在ER阳性胰腺癌表达低于阴性病例;ERC4mRNA在ER阴性胰腺癌表达明显低于阳性病例(P<0.05);ERD5mRNA在ER阳性胰腺癌表达低于阴性病例。结论ERα变异体的检出说明它们在胰腺癌发生过程中起一定的作用。在胰腺癌的发生过程中,ERα变异体mRNA的表达失控。
Objective To detect the expression of estrogen receptor-α (ER-α) protein and variants mRNA ERC4, ERD3, ERD5 in pancreatic cancer. Methods Expression of ER-α protein and variants mRNA-ERC4, ERD3, ERD5 in 50 cases of pancreatic cancer and 20 cases of mammary cancer was studied using the method of immunohistochemistry and RT-PCR, respectively. Results The positive rate of ER-α protein was 32%. The expression of ERD3 mRNA/ERD5 mRNA in ER ( + ) pancreatic cancer was lower than that in ER ( - ) pancreatic cancer, while the expression of ERC4mRNA in ER ( - ) pancreatic cancer was lower than that in ER ( + ) pancreatic cancer. Conclusions Abnormal expression of Mutant ER-α mRNA plays an important role in the development and progression of pancreatic cancer.
出处
《胰腺病学》
2004年第3期159-162,共4页
Chinese JOurnal of Pancreatology
基金
山东省卫生厅课题资助(2003-55)
