大肠癌组织P53蛋白与PCNA的表达及意义

Cocktail (P63+α-SMA)-labeling myoepithelium cell diagnose ductal carcinoma in situ and infiltrating breast carcinom

目的 研究P5 3蛋白及PCNA在结直肠肿瘤中的表达及意义。方法 使用结直肠手术切除标本 2 15例 ,全部作常规病理检查及免疫组化检查。使用P5 3与PCNA单抗即溶型试剂用S P法作酶标染色。结果 在高、中、低分化腺癌及DukesA、B、C分期中P5 3阳性率分别为 5 5 8%、70 6 %、95 5 % (P <0 0 0 1)及 6 6 7%、76 5 %、82 9% (P <0 0 5 )。PCNA强阳性率分别为 4 6 5 %、6 0 0 %、77 4 % (P <0 0 5 )及 4 1 7%、6 1 2 %、6 4 3% (P <0 0 1)。结论 P5 3及PCNA表达对癌组织的分化程度及显示病程进展转移的临床病理分期均呈正相关。

Objective To use P63, smooth muscle actin(α SMA), and cocktail(P63+α SMA)to label breast myoepithelium cells and investigate their roles in diagnosing ductal carcinoma in situ (DCIS) and infiltrating breast carcinoma Methods Myoepithelium cell staining was conducted in 6 cases of DCIS and 84 cases of infiltrating breast carcinoma Immunohistochemistry assay was used to detect the expression of P63, smooth muscle actin, and cocktail(P63+α SMA)in DCIS and infiltrating breast carcinoma tissues A paired t test and F test were used to evaluate whether the myoepithelium cell staining was significantly different Results The stain of myoepithelium cell layers by cocktail was more intense than either P63 or SMA alone The mean staining intensity was 6680, 6 38, and 5 97 for cocktail, SMA, and P63, respectively ( P =0 023 and 0 000 for cocktail vs SMA and P63) A total of 13.5% infiltrating breast carcinomas had positive P63 and cocktail staining Conclusion Cocktail labeling myoepithelium cells exhibit the highest sensitivity and specificity among the three methods. It can be used as an assisted approach to diagnose DCIS and infiltrating breast carcinoma