摘要
Aim: To study the DHT-binding ability of benign prostatic hypertrophy (BPH) and its etiological relationship with BPH. Methods: Cytosolic and nuclear fractions was obtained from 32 BPH tissues by superpubic prostatectomy and all the endogenous hormone were removed from the cytosolic and nuclear fractions by ether stripping. The content of the bound 3H-DHT was assayed after the addition of 3H-DHT. Results: The average DHT-binding capacity of the BPH is 0.024 nmol (protein)/g wet tissue. The DHT-binding capacity of the cytosolic and the nuclear fractions were (0.0128±0.0020) nmol/g and (0.0112±0.0059) nmol/g wet tissue, respectively, the difference of the two capacities being insignificant (P>0.05). Conclusion: The DHT-binding capacity of BPH is high and thus facilitates the effect of DHT on BPH.
