摘要
目的了解二期早发梅毒患者Th1/Th2和Tc1/Tc2平衡的变化。方法用三色法流式细胞术检测30例二期早发梅毒患者及20例正常人外周血Th、Tc细胞亚群。结果梅毒患者CD3+、 CD4+、NK细胞及CD4+/CD8+比值均明显低于对照组(P<0.001),而CD8+、CD19+细胞与对照组相比差异无显著性(P>0.05)。梅毒患者Th1细胞和Th1/Th2、Tc1/Tc2比值均明显低于对照组(P<0.001-0.01),但Tc0、Tc2细胞显著高于对照组(P<0.001)。结论二期梅毒患者存在细胞免疫功能紊乱,而体液免疫功能似无明显变化。Th及Tc细胞亚群向Th2和Tc2 细胞的极化及其相关的细胞因子可能与梅毒螺旋体清除不全和发生慢性感染有关。
Objective: To understand the change of Th1/Th2 and Tc1/Tc2 balance in patients with secondary early syphilis. Methods: Peripheral blood Th and Tc cell subpopu-lations were examined by three-color flow cytometry in 30 patients with secondary early syphilis and 20 healthy subjects. Results: Percentages of CD3+ CD4+ T cells and the ratio of CD4+CD8+ and NK cells in syphilitic patients were significantly lower than in the control group (P<0.001), but no statistical difference was found in percentages of CD8+ and CD19+ cells between the two groups (P > 0.05). The percentage of Th1 cells and ratios of Th1/Th2 and Tc1/Tc2 in syphilitic patients were clearly decreased as compared to those in controls (P<0.001-0.01), whereas percentages of Tc0 and Tc2 cells in syphilitics was higher than those in controls(P<0.001). Conclusion: There could be a disturbance of cellular immunity and the relatively intact function of humoral immunity in secondary syphilis. It is postulated that the polarization toward development of Th2 and Tc2 cells and their associated cytokines might be involved in the incomplete clearance of treponemes and the development of chronic infection.
基金
This item is sponsored by Public Health Bureau of Guangdong Province
