β-连环蛋白在毛囊周期中基因表达的实验研究

An experimental study on the gene expression ofβ-catenin in hair follicle cycle

目的:探讨 β-连环蛋白 ( β-catenin)mRNA在毛囊周期中的表达规律及意义。方法:在测定大鼠毛囊周期的基础上 ,应用RT-PCR方法检测毛囊周期不同阶段中 β-cateninmRNA表达的变化。结果:新生大鼠毛囊周期大约为28天 ,其中1～18天为生长期(anagen) ,19～22天为退化期(catagen) ,23～28天为静止期(telogen) ;β-catenin在生长早期毛囊中呈强表达 ,而 β-catenin在退化期和静止期表达微弱。结论:β-cateninmRNA在毛囊周期中呈规律性表达 ,提示其对毛囊的生长具有重要的促进作用 ,可能是毛囊生长。

Objective:To explore the expression and significance ofβ-catenin mRNA in hair follicle cycle.Methods:After the hair follicle cycles of wistar rats were definited,RT-PCR was used to detect the expression ofβ-catenin mRNA in different phages of hair follicle cycle.Results:The hair follicle cycle of wistar rats was last28d,composed of anagen(1～18)、catagen(19～22)and telogen(23～28).β-catenin mRNA was strongly expressed in early anagen,while weakly in catagen and telogen.Conclusion:The expression ofβ-catenin mRNA was altered regularly in hair follicle cycles,which suggestedβ-catenin might be an essential regulative factor in follicle formation and cycle maintenance.