IL-8和皮质醇与特发性早产关系的研究

A Study of the Relationship Among the Interleutic 8, Cortisol And Idiopathic Preterm Labor

目的 探讨白细胞介素 8和皮质醇含量的变化及其与特发性早产的关系。方法 随机选取 32例特发性早产孕妇作为病例组 ,及 15例同期正常孕妇作为对照组 ,采用ELISA法检测两组孕妇血清、羊水、尿液中的IL - 8含量 ,采用放射免疫法测定血清、羊水中的皮质醇含量。结果 早产组孕妇血清、羊水和尿液中IL - 8含量分别为 (0 .2 8± 0 .13) μg/ml,(0 .2 9± 0 .12 ) μg/ml和 0 .18± 0 .15× 10 -2g/mol肌酐 ,明显高于对照组的 (0 .16± 0 .0 7) μg/ml,(0 .17± 0 .0 9) μg/ml,(0 .0 5± 0 .0 2 ) g/mol肌酐 ;早产组孕妇血清和羊水中皮质醇含量分别为 (75 6 .38± 4 0 2 .35 ) μg/ml和 (96 .12± 89.4 9) μg/ml,明显高于对照组的 (5 12 .6 5± 14 3.2 3) μg/ml和 (37.10± 17.79) μg/ml。早产组孕妇血清、羊水中的IL - 8与皮质醇含量均呈正相关 (r1=0 .4 2 71,P <0 .0 5 ;r2 =0 .5 388,P <0 .0 5 )。结论 IL -

Objective To probe the relationships among the interleutic 8(IL-8),cortisol and idiopathic preterm labor. Methods The random sampling 32 pregnant women cases with idiopathic preterm labor were conducted as disease cases’ group, while 15 normal pregnant women acted as compared group.IL-8 concentrations in serum, amniotic fluid and urine of the two groups’ pregnant women were measured by enzyme-linked immunosorbant assay, and the cortisol concentration in the serum and amniotic fluid were surveyed by radio immunoassay. Results The concentrations of IL-8 in maternal serum, amniotic fluid and urine were significantly higher in preterm labor group than those in the compared group. [(0.28±0.13)μg/ml vs (0.16±0.07)μg/ml,(0.29±0.12)μg/ml vs (0.17±0.09)μg/ml,(0.18±0.15)×10^(-2)g/mol Cr vs (0.05±0.02)g/mol Cr;P<0.05,P<0.05,P<0.01,respectively]. The concentrations of cortisol in maternal serum, amniotic fluid were significantly higher in preterm labor group than those in the compared group.[(756.38±402.35)μg/ml vs (512.65±143.23)μg/ml,(96.12±89.49)μg/ml vs (37.10±17.79)μg/ml;P<0.01,P<0.01,respectively]. A plus correlation was found between IL-8 and cortisol contractions in the serum or amniotic fluid of the preterm labor group(r 1=0.4271,P<0.05;r 2=0.5388,P<0.05). Conclusions The concentrations of IL-8 and cortisol play an important role in the mechanisms of idiopathic preterm labor.