摘要
α-转移葡萄糖苷酶是生产低聚异麦芽糖的关键酶。本文成功选育一株高产α-转移葡萄糖苷酶菌株ASP-2。并对该菌株进行了固态发酵条件优化,结果表明:麸皮和水的质量比为1:1,35℃培养48h时产酶量及酶活较高,ASP-2菌株生长的最适pH值为6.0,碳源和氮源不是该菌株在此培养基中产酶的必需元素,从降低生产成本的角度考虑,可以不用。
Alpha-transglucosidase is a critical enzyme of producing isomaltooligosaccharide. In this paper, strain ASP-2 ofproducing α-transglucosidase was obtained by screening experiments. The optimum conditions for fermentation in solid-statewere:bran:water(m/m) =1:1,culture temperature and duration were 35℃ and 48h respectively, the optimum pH value was 6.0.Carbon sources and nitrogen sources were not necessary nutrition for producing enzyme in this culture medium , so they needn'tbe added in the culture medium in order to reduce production cost.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2004年第10期49-53,共5页
Food Science
