大豆异黄酮糖苷转化酶产生菌株的筛选及培养条件的研究

Study on Screening Strain and Cultural Conditions of Strain Obtained by Soybean Isoflavones Glycosides Transform Enzyme

通过菌株筛选,确定了黑曲霉3866为糖苷转化酶高产菌株。产酶最适培养基配方为:麸皮4%, (NH4)2SO40.1%,KH2PO4 0.1%,抗坏血酸0.1%,MgSO4 0.1%,水杨苷0.05%。产酶的最佳培养条件为:发酵起始pH值6.0,发酵温度30℃,摇床160r/min,发酵时间84h。酶活力最高可达152.45U。

Aspergillus niger 3866 is confirmed the highy enzyme activity producing strain by screening strain .The prescriptionof best medium of producing enzyme is: Bran is 4%, (NH4)2SO4 0.1%, KH2PO4 0.1%, ascorbic acid 0. 1%, MgSO4 0.1%,salicinis 0. 05%. The best cultivation condition of producing enzyme is pH 6. 0, 30℃, 160 r/min, 84 h. Enzyme vigor can reach as highas 152.45U.