应用组织微阵列技术检测肝癌中血管内皮生长因子及P16和P53的表达及意义

Detection of expression of VEGF, P16 and P53 in HCC by tissue microarray assay and its significance

目的 利用组织芯片技术检测肝癌中血管内皮生长因子及P16 ,P5 3的表达 ,初步探讨血管内皮生长因子及P16 ,P5 3的表达在肝细胞癌发生机制以及预后评估方面的作用。方法 采用免疫组化技术结合临床病理资料检测 6 1例肝癌和 5 0例癌旁组织的VEGF及P16 ,P5 3的表达情况。结果 VEGF ,P16和P5 3的表达与肿瘤的恶性程度密切相关。在癌旁组织中 ,P16蛋白全表达 ,而P5 3蛋白全不表达。随着肿瘤恶性程度的提高 ,P16表达降低而P5 3表达水平增高 ,VEGF亦随着癌变进程表达逐渐增高 ,差异非常显著 (P <0 0 0 1)。在不同侵袭转移倾向组中三者的表达也存在着显著性差异 (P <0 0 5 )。三者的相关性分析提示VEGF和P16之间存在负相关 (r=- 0 72 3,P <0 0 5 ) ;VEGF和P5 3之间存在正相关 (r=0 36 4 ,P >0 0 5 ) ,P16和P5 3之间存在负相关 (r=- 0 2 78,P >0 0 5 ) ,但相关不显著。结论 应用组织芯片高效检测临床组织样本具有快速、方便、经济、准确的优点。VEGF及P16 ,P5 3的异常表达可能与肝癌的发生发展、侵袭转移有密切关系。

Objective To detect the expression of VEGF, P16 and P53 in HCC by tissue microarray assay. Methods The expression of VEGF, P16 and P53 was detected with immunohistochemistry and clinicopathological examination in 60 specimens of HCC and 50 specimens of peritumorous tissue. Results The expression of VEGF, P16 and P53 were correlated with histological differentiation and potential of invasion and metastasis of HCC. Conclusions The expression of VEGF, P16 and P53 might be related to the development, invasion and metastasis of HCC. It is feasible to use tissue microarray assay for rapid, economic and accurate screening of clinical tissue specimens on a large scale.